Kobayashi M, Yanaka N, Nagasawa T, Yamada H
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
J Antibiot (Tokyo). 1990 Oct;43(10):1316-20. doi: 10.7164/antibiotics.43.1316.
Using resting cells of Rhodococcus rhodochrous J1, in which a large amount of nitrilase is induced, a simple and efficient bioconversion process for the production of pyrazinoic acid, an antimycobacterial agent, through catalysis by a nitrilase was developed. The reaction conditions for production of pyrazinoic acid were optimized. Under optimum conditions, 3.5 M cyanopyrazine was converted to pyrazinoic acid, with a molar conversion yield of 100%. The highest yield achieved corresponded to 434 g of pyrazinoic acid per liter of reaction mixture. The synthesized pyrazinoic acid was isolated and identified physico-chemically.
利用大量诱导产生腈水解酶的红平红球菌J1的静息细胞，开发了一种简单高效的生物转化过程，通过腈水解酶催化生产抗分枝杆菌剂吡嗪酸。优化了吡嗪酸的反应条件。在最佳条件下，3.5M氰基吡嗪转化为吡嗪酸，摩尔转化率为100%。所达到的最高产量相当于每升反应混合物434克吡嗪酸。对合成的吡嗪酸进行了分离，并通过物理化学方法进行了鉴定。
