Doi Y, Segawa A, Kawaguchi Y, Kunioka M
Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.
FEMS Microbiol Lett. 1990 Jan 15;55(1-2):165-9. doi: 10.1016/0378-1097(90)90188-v.
The cyclic process of biosynthesis and degradation of poly(3-hydroxyalkanoate) (PHA) was studied in Alcaligenes eutrophus under conditions of nitrogen-limitation of growth. A. eutrophus cells, which had accumulated poly(3-hydroxybutyrate) (PHB) of 55 wt% content within cells from butyric acid, were transferred into a nitrogen-free medium containing pentanoic acid as the sole carbon source and cultivated at 30 degrees C and pH 7.5. The content of PHB in A. eutrophus cells decreased with time, whereas a copolyester of 3-hydroxybutyrate (HB) and 3-hydroxyvalerate (HV) units, P(HB-co-HV), was accumulated in the presence of pentanoic acid. Conversely, when A. eutrophus cells with 50 wt% content of P(HB-co-56% HV) were incubated in a nitrogen-free medium containing butyric acid, the content of P(HB-co-56% HV) decreased with time, whereas PHB was accumulated. These results indicate the cyclic nature of PHA metabolism in A. eutrophus under these conditions.
在生长氮限制条件下，研究了真养产碱杆菌中聚（3-羟基链烷酸酯）（PHA）生物合成和降解的循环过程。将已从丁酸中在细胞内积累了含量为55 wt%的聚（3-羟基丁酸酯）（PHB）的真养产碱杆菌细胞转移到含有戊酸作为唯一碳源的无氮培养基中，并在30℃和pH 7.5下培养。真养产碱杆菌细胞中PHB的含量随时间下降，而在戊酸存在下积累了由3-羟基丁酸酯（HB）和3-羟基戊酸酯（HV）单元组成的共聚酯P（HB-co-HV）。相反，当将含有50 wt% P（HB-co-56% HV）的真养产碱杆菌细胞在含有丁酸的无氮培养基中培养时，P（HB-co-56% HV）的含量随时间下降，而积累了PHB。这些结果表明在这些条件下真养产碱杆菌中PHA代谢的循环性质。
