Shi Yingwu, Zhang Xuebing, Lou Kai
Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
Wei Sheng Wu Xue Bao. 2012 Oct 4;52(10):1297-308.
The aim of this study was to investigate the composition and distribution variation of endophytic bacteria and fungi in Achnatherum inebrians.
The DNA of seed, leaf, stem and root was extracted with liquid nitrogen grinding method. Then, 16S rDNA and Internally Transcribed Spacer (ITS) sequence were digested with restriction enzymes Hha I , Rsa I and Hhae III, Hinf I to obtain terminal restriction fragments. The terminal restriction fragments were matched to bacterial and fungal genera by the T-RFLP Analysis Program, and the community component and similarity of endophyte in Achnatherum inebrians were analyzed.
The diversity of endophytic bacteria and fungi was the most abundant in root and seed of Achnatherum inebrians. All the predominant bacterial population was Bacillus (above 29%) in different organs of Achnatherum inebrians. The predominant fungal population was Mycosphaerella (6.5%), Teratosphaeria (4.5%), Fragum (1.1%), Sebacina (11.3%) in seed, leaf, stem and root, respectively. The structure of the bacterial communities in the stem and leaf were similar, whereas the structure of the bacterial communities in the seed and other tissue were different. The structure of the fungi communities in the stem and seed were similar, whereas the structure of the fungi communities in the leaf and other tissue were different.
There was abundant endophytic microbial diversity in Achnatherum inebrians.
本研究旨在探究醉马草内生细菌和真菌的组成及分布变化。
采用液氮研磨法提取种子、叶片、茎和根的DNA。然后，用限制性内切酶Hha I、Rsa I和Hhae III、Hinf I对16S rDNA和内转录间隔区（ITS）序列进行酶切以获得末端限制性片段。通过T-RFLP分析程序将末端限制性片段与细菌和真菌属进行匹配，分析醉马草内生菌的群落组成及相似性。
醉马草的根和种子中内生细菌和真菌的多样性最为丰富。在醉马草的不同器官中，所有主要细菌种群均为芽孢杆菌（超过29%）。种子、叶片、茎和根中主要真菌种群分别为球腔菌属（6.5%）、球座菌属（4.5%）、盘菌属（1.1%）、角担菌属（11.3%）。茎和叶中细菌群落结构相似，而种子和其他组织中细菌群落结构不同。茎和种子中真菌群落结构相似，而叶和其他组织中真菌群落结构不同。
醉马草中存在丰富的内生微生物多样性。
