Miyamachi K
Department of Neurosurgery, Hokkaido University, School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1990 Jan;65(1):41-9.
The 6-day subrenal capsule assay for determining chemotherapeutic sensitivities of brain tumors was studied. Rat glioma 9L and ACNU resistant 9L-2 were transplanted under the renal capsule of normal immunocompetent WKA rats for laboratory investigation. Evaluation of implanted tumor growth till 12 days was performed. The effects of chemotherapeutic agents administered intravenously were evaluated by measuring the growth rate of implanted tumor specimens. The results obtained from SRC were compared with the results from colony forming assay. Both were correlated to each other. On the other hand, histological investigation revealed that implanted human tumor cells had been diminished and implanted tumor was replaced by immunoreactive cells from the host in many cases. These results threw doubt on a reliability of SRC. To avoid this immunoreaction, cyclophosphamide was injected as immunosuppressive agent subcutaneously 24 hours before implantation. In such cases, the growth rates of implanted tumors were increased and histologically the implanted tumor cells existed for 6 days after implantation. Twenty-three malignant brain tumors (malignant astrocytomas 16, metastatic tumors 5, malignant lymphoma 2) were obtained as surgical specimens. Evaluable assay rate of our study were 89%. 15 patients with malignant astrocytomas were studied about correlation between the sensitivities of ACNU and post-operative clinical courses. Overall clinical correlation of 15 cases of malignant astrocytomas was 47%. These results from subrenal capsule assay are not seemed to be beneficial for clinical use. Immunoreactive response when using immunocompetent rats must be solved in future.
研究了用于确定脑肿瘤化疗敏感性的6天肾包膜下试验。将大鼠胶质瘤9L和对ACNU耐药的9L-2移植到具有正常免疫活性的WKA大鼠的肾包膜下进行实验室研究。评估植入肿瘤直至12天的生长情况。通过测量植入肿瘤标本的生长速率来评估静脉注射化疗药物的效果。将从肾包膜下试验获得的结果与集落形成试验的结果进行比较。两者相互关联。另一方面，组织学研究表明，在许多情况下，植入的人类肿瘤细胞减少，植入的肿瘤被宿主的免疫反应性细胞所取代。这些结果对肾包膜下试验的可靠性提出了质疑。为避免这种免疫反应，在植入前24小时皮下注射环磷酰胺作为免疫抑制剂。在这种情况下，植入肿瘤的生长速率增加，组织学上植入的肿瘤细胞在植入后6天仍然存在。获取了23例恶性脑肿瘤（恶性星形细胞瘤16例、转移瘤5例、恶性淋巴瘤2例）作为手术标本。我们研究的可评估试验率为89%。对15例恶性星形细胞瘤患者进行了ACNU敏感性与术后临床病程之间相关性的研究。15例恶性星形细胞瘤的总体临床相关性为47%。肾包膜下试验的这些结果似乎对临床应用没有益处。未来必须解决使用具有免疫活性的大鼠时的免疫反应问题。
