Faculty of Biotechnology, University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania.
J Med Food. 2013 Feb;16(2):155-66. doi: 10.1089/jmf.2012.0030. Epub 2013 Jan 5.
The antioxidant and antimicrobial properties of two edible mushrooms (Pleurotus ostreatus PBS281009 and Coprinus comatus M8102) were evaluated in this study. The mycelium ethanolic extract obtained in the presence of four different nitrogen sources was investigated with regard to their production of phenolic compounds, β-carotene, lycopene, and their antimicrobial and antioxidant properties. Significant differences in phenol and β-carotene concentrations in mycelia grown in different nitrogen sources were observed. Corn extract proved to be the most appropriate source for increasing bioactive properties, leading to the lowest EC₅₀ and minimum inhibitory concentration values in the P. ostreatus PBS281009 species, while peptone was the most suitable for the C. comatus M8102 species. The antimicrobial capacity was screened against Gram-positive and Gram-negative bacteria and against two species of Candida. These results indicate that the mycelia from these two edible mushrooms can be used as nutraceuticals and functional products.
本研究评估了两种食用蘑菇（糙皮侧耳 PBS281009 和墨汁鬼伞 M8102）的抗氧化和抗菌特性。研究了在四种不同氮源存在下获得的菌丝体乙醇提取物，考察了其酚类化合物、β-胡萝卜素、番茄红素的产生及其抗菌和抗氧化特性。在不同氮源中生长的菌丝体的酚和 β-胡萝卜素浓度存在显著差异。玉米提取物被证明是最适合提高生物活性特性的氮源，导致糙皮侧耳 PBS281009 物种的 EC₅₀ 和最小抑菌浓度值最低，而蛋白胨是最适合墨汁鬼伞 M8102 物种的氮源。抗菌能力针对革兰氏阳性菌和革兰氏阴性菌以及两种念珠菌进行了筛选。这些结果表明，这两种食用蘑菇的菌丝体可用作营养保健品和功能性产品。
