Yu Da-liang, Li Xiao-mei, Wang Xi-mei, Wang Xiao-qiu, Li Xiang-pei
Department of Rheumatology & Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China.
Zhonghua Yi Xue Za Zhi. 2012 Oct 23;92(39):2775-7.
To detect the expression of B7-H4 in salivary gland and sera in patients with primary Sjogren's syndrome (pSS).
A total of 40 pSS patients were referred to our department from June 2009 to January 2011. Immunohistochemistry and flow cytometry were used to detect the expression of B7-H4 in salivary gland from pSS patients and disease controls. Enzyme-linked immunosorbent assay (ELISA) was used to detect soluble B7-H4 of serum from pSS patients and healthy donors.
Immunohistological staining revealed that B7-H4 antigen was restricted to tubular epithelium. The B7-H4 positive expression of tubules in salivary gland biopsies from pSS patients (18 ± 14)% were lower than that of controls (85 ± 13)% (P < 0.05). Flow cytometry revealed that the B7-H4 expression of cell suspensions from salivary gland from pSS patients (42 ± 21)% were lower than that of controls (48 ± 22)% (P < 0.01). And the serum level of soluble B7-H4 detected in pSS patients (49 ± 31)µg/L significantly decreased than that in healthy donors (71 ± 27) µg/L (P < 0.05) and positively correlated with saliva and tear flow rates (P < 0.01) respectively.
The expression of B7-H4 molecule may play some roles in the progression of pSS. And a further understanding of its mechanism helps to elucidate the pathogenesis of pSS.
检测原发性干燥综合征（pSS）患者唾液腺及血清中B7-H4的表达。
2009年6月至2011年1月共40例pSS患者被转诊至我科。采用免疫组织化学和流式细胞术检测pSS患者及疾病对照唾液腺中B7-H4的表达。采用酶联免疫吸附测定（ELISA）检测pSS患者及健康供体血清中可溶性B7-H4。
免疫组织化学染色显示B7-H4抗原局限于小管上皮。pSS患者唾液腺活检小管的B7-H4阳性表达率为（18±14）%，低于对照组的（85±13）%（P<0.05）。流式细胞术显示，pSS患者唾液腺细胞悬液中B7-H4表达率为（42±21）%，低于对照组的（48±22）%（P<0.01）。pSS患者血清中可溶性B7-H4水平（49±31）μg/L明显低于健康供体（71±27）μg/L（P<0.05），且分别与唾液及泪液流速呈正相关（P<0.01）。
B7-H4分子的表达可能在pSS进展中起一定作用。进一步了解其机制有助于阐明pSS的发病机制。
