利用Verticillium sp. GF39 细胞将芳香族 N-杂环化合物氧化为 N-氧化物。
Oxidation of aromatic N-heterocyclic compounds to N-oxides by Verticillium sp. GF39 cells.
机构信息
Department of Biomolecular Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
出版信息
J Biosci Bioeng. 2013 Jun;115(6):651-3. doi: 10.1016/j.jbiosc.2012.12.002. Epub 2013 Jan 3.
Verticillium sp. GF39, catalyzing the oxidation of 1-methylisoquinoline to 1-methylisoquinoline N-oxide, was found to be the highest N-oxide producer. Under the optimized reaction conditions, the whole cells of Verticillium sp. GF39 formed 5 mM 1-methylisoquinoline N-oxide from 1-methylisoquinoline with a molar conversion yield of 100% after a 10-h incubation at 20°C. The whole cells also acted on pyridine, 2-methylpyridine, quinoline and isoquinoline and formed the corresponding N-oxides.
从 1-甲基异喹啉到 1-甲基异喹啉 N-氧化物的氧化，被发现是最高的 N-氧化物生产。在优化的反应条件下，经过 10 小时 20°C 孵育，青霉菌 GF39 的整个细胞从 1-甲基异喹啉形成 5 mM 的 1-甲基异喹啉 N-氧化物，摩尔转化率为 100%。整个细胞还作用于吡啶、2-甲基吡啶、喹啉和异喹啉，形成相应的 N-氧化物。
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