Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, PR China.
Bioorg Med Chem Lett. 2013 Feb 1;23(3):630-4. doi: 10.1016/j.bmcl.2012.12.005. Epub 2012 Dec 14.
In our previous studies, geraniin was reported to have a preventive effect in the rat model of tretinoin-induced osteoporosis. However, whether geraniin exhibits an inhibitory effect on bone resorption or on MMP-9 expression is not yet known. We present here our novel findings from in vitro experiments that geraniin (a) decreases the number of mature osteoclasts and pre-osteoclast in cultures, (b) reduces the osteoclastic fusion index, and (c) inhibits the resorption areas and resorption pits. We also report that geraniin suppresses the mRNA and protein expression levels of MMP-9. These results demonstrate that geraniin has an inhibitory effect on the bone-absorption ability of osteoclasts in vitro, and the mechanisms may be closely associated with the downregulation of mRNA and protein expression of MMP-9.
在我们之前的研究中，报告称 geraniin 对维甲酸诱导的骨质疏松症大鼠模型具有预防作用。然而，geraniin 是否对骨吸收或 MMP-9 表达具有抑制作用尚不清楚。我们在此介绍了我们的新发现，即 geraniin（a）减少培养物中成熟破骨细胞和前破骨细胞的数量，（b）降低破骨细胞融合指数，（c）抑制吸收面积和吸收坑。我们还报告说，geraniin 抑制 MMP-9 的 mRNA 和蛋白表达水平。这些结果表明，geraniin 对体外破骨细胞的骨吸收能力具有抑制作用，其机制可能与 MMP-9 的 mRNA 和蛋白表达下调密切相关。
