Yi Zhibiao, Ma Lin, Wang Baoqiang, Cai Wenxuan, Xiang Zheng
Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan, P.R. China.
GuangDong Province Hospital of Traditional Chinese Medicine, Guangzhou, P.R. China.
Curr Pharm Anal. 2017 Aug;13(4):398-402. doi: 10.2174/1573412912666160527125937.
Geraniin has many biological activities including anti-osteoporotic and anti-hyperglycemic efficacies.
A rapid and simple method for the determination of geraniin in rat plasma using ultra performance liquid chromatography coupled to ultraviolet detector was developed. The plasma sample, spiked with epicatechin as an internal standard, was subjected to ethyl acetate extraction prior to analysis. Chromatographic separation was performed on the HSS T3 column and monitored at a wavelength of 280 nm. The limit of detection and lower limit of quantification was 0.07 μg/mL and 0.2 μg/mL in rat plasma, respectively.
Good linearity was obtained in the range of 0.2 - 200 μg/mL, and the correlation coefficient was better than 0.997. The intra-day and inter-day precisions decreased 9.8%. The accuracy of QC samples ranged from 84.4% to 87.1%. The extraction recovery ranged from 88.4% to 90.3% and the matrix effect ranged from 84.4% to 87.2%. The analyte was stable in rat plasma when stored at room temperature for 12 hours, 4°C for 24 hours and -20°C for 15 days. t1/2 and t1/2 for i.v. was 0.21 ± 0.10 and 7.20 ± 2.20 h, respectively. Plasma clearance (CL) was 0.03 ± 0.02 L/h/kg and apparent volume of distribution (Vz) was 0.05 + 0.01 L/kg. The developed method was successfully applied to the pharmacokinetic study of geraniin in rats.
老鹳草素具有多种生物活性，包括抗骨质疏松和抗高血糖功效。
建立了一种快速简便的超高效液相色谱-紫外检测法测定大鼠血浆中的老鹳草素。血浆样品加入表儿茶素作为内标，在分析前用乙酸乙酯萃取。采用HSS T3柱进行色谱分离，检测波长为280 nm。大鼠血浆中的检测限和定量下限分别为0.07μg/mL和0.2μg/mL。
在0.2 - 200μg/mL范围内线性良好，相关系数优于0.997。日内和日间精密度下降9.8%。质量控制样品的准确度在84.4%至87.1%之间。萃取回收率在88.4%至90.3%之间，基质效应在84.4%至87.2%之间。分析物在大鼠血浆中于室温下储存12小时、4℃下储存24小时和-20℃下储存15天时均稳定。静脉注射的t1/2和t1/2分别为0.21±0.10和7.20±2.20小时。血浆清除率（CL）为0.03±0.02 L/h/kg，表观分布容积（Vz）为0.05 + 0.01 L/kg。所建立的方法成功应用于大鼠老鹳草素的药代动力学研究。
