Liebich H M, Bubeck J I, Pickert A, Wahl G, Scheiter A
Medizinische Universitätsklinik, Tübingen, F.R.G.
J Chromatogr. 1990 Feb 2;500:615-27. doi: 10.1016/s0021-9673(00)96096-5.
Hippuric acid (HA) and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (FA) were determined in serum, plasma, ultrafiltrate and urine by gas chromatography (GC), high-performance liquid chromatography and GC with mass-selective detection, and the methods were compared. As determined by affinity chromatography and analysis of serum and ultrafiltrate, 0.5% of FA in serum occurs free and 99.5% is bound to albumin. In haemodialysed patients with chronic renal failure, the plasma levels of HA and FA are elevated in comparison with normal controls and hospital patients without kidney diseases: HA, 11.1 +/- 5.7 mg/dl (n = 86); FA, 1.9 +/- 1.2 mg/dl (n = 86). Gradual increases in HA in serum, depending on the creatinine concentrations, are found in non-dialysed patients with chronic renal failure. By haemodialysis and haemofiltration the HA levels are lowered (53-66 and 30-36%, respectively), whereas FA is not dialysable.
采用气相色谱法（GC）、高效液相色谱法以及带质量选择性检测的气相色谱法对血清、血浆、超滤液和尿液中的马尿酸（HA）和3-羧基-4-甲基-5-丙基-2-呋喃丙酸（FA）进行了测定，并对这些方法进行了比较。通过亲和色谱以及血清和超滤液分析确定，血清中0.5%的FA以游离形式存在，99.5%与白蛋白结合。在接受血液透析的慢性肾衰竭患者中，与正常对照组以及无肾脏疾病的住院患者相比，其血浆中HA和FA的水平升高：HA为11.1±5.7mg/dl（n = 86）；FA为1.9±1.2mg/dl（n = 86）。在未接受透析的慢性肾衰竭患者中，血清中的HA会根据肌酐浓度逐渐升高。通过血液透析和血液滤过，HA水平会降低（分别降低53 - 66%和30 - 36%），而FA不可透析。
