College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
Appl Biochem Biotechnol. 2013 Feb;169(3):923-40. doi: 10.1007/s12010-012-0026-9. Epub 2013 Jan 6.
Active components from traditional Chinese medicine (TCM) are the material basis for disease treatment. Extraction, identification, and transformation are the critical processes to analyze and use the active components of TCM. Botanic TCM takes up 90% of total Chinese crude drugs. Some active components are complex and of lower level; moreover, most of them are enveloped under plant cell wall. Traditional extraction methods such as lixiviate method, decoction, and others are often hindered by cell wall, leading to low extraction efficiency, low clearance of impurity, and other problems, which have restricted the development of TCM. This paper reviews both domestically and internationally published literatures in recent years on application of enzymatic methods in the extraction and transformation of active ingredients from TCM. Principles of enzymatic method and its application in extraction and transformation of active ingredients and in dreg recycles of TCM are introduced in detail. With the development of TCM modernization, enzymatic method applied in the domain of TCM has achieved prominent benefits, not only improving the extraction and separation rate of active ingredients from TCM and elevating the transformation level and production, but also reducing costs in the transformation of active ingredients.
中药（TCM）的活性成分是治疗疾病的物质基础。提取、鉴定和转化是分析和利用中药活性成分的关键过程。植物性中药占中国天然药物总量的 90%。一些活性成分复杂且含量较低；此外，它们中的大多数被植物细胞壁包裹。传统的提取方法，如浸提法、煎煮法等，往往受到细胞壁的阻碍，导致提取效率低、杂质去除率低等问题，限制了中药的发展。本文综述了近年来国内外关于酶法在中药活性成分提取和转化中的应用。详细介绍了酶法的原理及其在中药活性成分提取转化和中药渣循环中的应用。随着中药现代化的发展，酶法在中药领域的应用取得了显著的效益，不仅提高了中药活性成分的提取分离率，提高了转化水平和产量，而且降低了活性成分转化的成本。
