Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Lett Appl Microbiol. 2013 Mar;56(3):216-21. doi: 10.1111/lam.12036. Epub 2013 Jan 7.
Of the 13 serotypes, 4b serotype strains are responsible for the majority of recorded invasive listeriosis outbreaks, although some recent listeriosis outbreaks have been attributed to strains of serotypes 1/2a and 1/2b. Virulence and response to osmotic stress in 41 Listeria monocytogenes strains representing serotypes 1/2a, 1/2b and 4b was investigated. It was found that serotype 4b and 1/2b strains exhibited highest invasion efficiency and formed largest plaques in HT-29 cell monolayer. Invasiveness in response to 10-min exposure to 0·3 mol l⁻¹ NaCl was the highest in serotype 4b strains. We demonstrated that 4b serotype L. monocytogenes strains not only have the greatest pathogenic potential but also are the most invasive in response to salt stress.
Listeria monocytogenes 4b serotype strains are responsible for the majority of recorded invasive listeriosis outbreaks. We showed that strains of serotype 4b are not only the most virulent L. monocytogenes strains but also have the best capacity to enhance their invasiveness in response to salt stress. Our results suggest possession of effective stress response mechanisms of 4b serotype strains, which may contribute to the high infection potential of this subpopulation.
在 13 种血清型中，4b 血清型菌株是导致大多数记录在案的侵袭性李斯特菌病暴发的主要原因，尽管最近一些李斯特菌病暴发归因于 1/2a 和 1/2b 血清型的菌株。本研究调查了代表血清型 1/2a、1/2b 和 4b 的 41 株李斯特菌单核细胞增生李斯特氏菌的毒力和对渗透压胁迫的反应。结果发现，4b 血清型和 1/2b 血清型菌株表现出最高的入侵效率，并在 HT-29 细胞单层中形成最大的噬菌斑。在 0.3mol l⁻¹ NaCl 中暴露 10 分钟时，4b 血清型菌株的侵袭性最高。我们证明，4b 血清型李斯特菌不仅具有最大的致病潜力，而且在盐胁迫下的侵袭性最强。
李斯特菌 4b 血清型菌株是导致大多数记录在案的侵袭性李斯特菌病暴发的主要原因。我们表明，4b 血清型菌株不仅是最毒力的李斯特菌菌株，而且具有在盐胁迫下增强其侵袭性的最佳能力。我们的结果表明，4b 血清型菌株具有有效的应激反应机制，这可能有助于该亚群的高感染潜力。
