Mitov B
Vet Med Nauki. 1979;16(10):96-102.
The location of the rotaviral precipitating antigen and the possibility for its production from cattle rotaviral strains adapted on cell cultures of calf kidney were investigated. Highest titer antigen was produced by concentration with ammonium sulfate simultaneously from the maintaining medium and from the cell monolayer. Comparative studies on the antigenic and physico-chemical properties of the cell-cultural and the faeces rotaviral precipitating antigen were made. The identity of both antigens was proven by the immunodiffuse reaction and by their resistance to ether and tripsine. A considerably higher temperature sensitivity was established of the cell-cultural precipitating antigen as compared to that from faeces. The precipitating antigen was used for confirming the presence of antirotaviral antibodies in the serums of cattle, man and sheep.
对轮状病毒沉淀抗原的位置以及能否从适应小牛肾细胞培养的牛轮状病毒株中产生该抗原进行了研究。通过用硫酸铵同时从维持培养基和细胞单层中进行浓缩，可产生最高滴度的抗原。对细胞培养的轮状病毒沉淀抗原和粪便轮状病毒沉淀抗原的抗原性及物理化学性质进行了比较研究。通过免疫扩散反应以及它们对乙醚和胰蛋白酶的抗性，证明了两种抗原的一致性。与粪便中的沉淀抗原相比，已确定细胞培养的沉淀抗原对温度更为敏感。该沉淀抗原用于确认牛、人和羊血清中抗轮状病毒抗体的存在。
