Almeida J D, Hall T, Banatvala J E, Totterdell B M, Chrystie I L
J Gen Virol. 1978 Jul;40(1):213-8. doi: 10.1099/0022-1317-40-1-213.
It has been found that 1000-fold more bovine rotavirus is obtained when trypsin is incorporated in the maintenance medium and allowed to remain throughout the growth cycle. This holds true for primary calf kidney (CK) cells and also for several continuous and semi-continuous cell lines. In the presence of trypsin it has been possible to pass the virus serially on continuous cell lines seven times. Concentrations of 1 to 10 microgram/ml of trypsin are found to be effective. Preliminary results suggest that the same technique will be effective for the in vitro propagation of human rotavirus.
已发现，当在维持培养基中加入胰蛋白酶并使其在整个生长周期中保持存在时，可获得比原来多1000倍的牛轮状病毒。这对于原代小牛肾（CK）细胞以及几种连续和半连续细胞系均适用。在胰蛋白酶存在的情况下，已能够在连续细胞系上连续传代该病毒7次。发现1至10微克/毫升的胰蛋白酶浓度是有效的。初步结果表明，相同技术对人轮状病毒的体外增殖也将有效。
