Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610, Prague 6, Czech Republic.
Amino Acids. 2013 Apr;44(4):1095-105. doi: 10.1007/s00726-012-1449-x. Epub 2013 Jan 8.
Oostatic peptides are organic molecules, which influence an insect reproduction due to a regulation of the eggs development. It was proved that decapeptide-H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Pro-Pro-Pro-OH (YDPAPPPPPP)-isolated from mosquito Aedes aegypti, inhibits trypsin activity in the midgut of the mosquito. Therefore, it was named trypsin-modulating oostatic factor (Aea-TMOF). Feeding the recombinant cells with cloned and expressed TMOF on the coat protein of tobacco mosaic virus (TMV) to mosquito larvae, caused larval mortality. The TMOF was therefore designed for usage as a new biorational insecticide against mosquito. Similarly, a hexapeptide-H-Asn-Pro-Thr-Asn-Leu-His-OH (NPTNLH)-was isolated from the grey flesh fly Neobellieria bullata. This peptide and some of its analogs inhibited trypsin-like synthesis by the midgut in female flies and was therefore entitled Neb-TMOF. Interestingly, the synthetic Aea-TMOF and mainly its C-terminus shorten analogs, including those containing D-amino acids or methylene-oxy isosteric bond, quickly and strongly inhibited the hatchability and egg development in the flesh fly N. bullata.
卵泌肽是一类有机分子，通过调节昆虫卵子的发育来影响昆虫的繁殖。已证实，从埃及伊蚊中分离出的十肽-H-Tyr-Asp-Pro-Ala-Pro-Pro-Pro-Pro-Pro-Pro-OH (YDPAPPPPPP) 抑制了蚊子中肠的胰蛋白酶活性。因此，它被命名为胰蛋白酶调节卵泌因子 (Aea-TMOF)。将克隆和表达的 TMOF 融合到烟草花叶病毒 (TMV) 的外壳蛋白上，喂食给蚊子幼虫的重组细胞，导致幼虫死亡。因此，TMOF 被设计用作新型生物理性杀虫剂来防治蚊子。同样，从灰色肉蝇 Neobellieria bullata 中分离出六肽-H-Asn-Pro-Thr-Asn-Leu-His-OH (NPTNLH)。这种肽及其一些类似物抑制了雌性苍蝇中肠的胰蛋白酶样合成，因此被命名为 Neb-TMOF。有趣的是，合成的 Aea-TMOF 及其主要的 C 末端缩短类似物，包括含有 D-氨基酸或亚甲基氧等排体的类似物，能够快速而强烈地抑制肉蝇 N. bullata 的孵化率和卵子发育。
