从灰肉蝇（新贝氏丽蝇，即麻蝇属）卵巢中对胰蛋白酶调节卵发育抑制因子（TMOF）进行测序和特性分析。

Sequencing and characterization of trypsin modulating oostatic factor (TMOF) from the ovaries of the grey fleshfly, Neobellieria (Sarcophaga) bullata.

作者信息

Bylemans D, Borovsky D, Hunt D F, Shabanowitz J, Grauwels L, De Loof A

机构信息

Zoological Institute, Katholieke Universiteit Leuven, Belgium.

出版信息

Regul Pept. 1994 Feb 3;50(1):61-72. doi: 10.1016/0167-0115(94)90192-9.

DOI:10.1016/0167-0115(94)90192-9

PMID:8159807

Abstract

Injection of crude extracts of late vitellogenic ovaries into staged females of the grey fleshfly Neobellieria (Sarcophaga) bullata inhibited oocyte development and biosynthesis of trypsin-like enzymes in the gut. Trypsin synthesis in N. bullata is cyclic and is correlated with egg development, which is discontinuous. A trypsin modulating oostatic factor (Neb-TMOF) was purified from 10,000 vitellogenic ovaries and sequenced by mass spectrometry. Neb-TMOF is a hexapeptide (NH2-NPTNLH-COOH). Injection of the hormone at physiological concentrations (10(-9) M), inhibited trypsin-like synthesis by the midgut of liver-fed female flies, and caused a reduction of the vitellogenin concentration in the hemolymph and of oocyte growth. The role of Neb-TMOF in controlling egg development and the physiological similarities with Aedes-TMOF are discussed.

摘要

将晚期卵黄发生期卵巢的粗提物注射到处于特定发育阶段的灰肉蝇（新钟蝇属（肉蝇属））雌性体内，会抑制卵母细胞发育以及肠道中类胰蛋白酶的生物合成。灰肉蝇体内的胰蛋白酶合成是周期性的，且与不连续的卵子发育相关。从10000个卵黄发生期卵巢中纯化出一种胰蛋白酶调节性卵发育抑制因子（Neb-TMOF），并通过质谱法对其进行了测序。Neb-TMOF是一种六肽（NH2-NPTNLH-COOH）。以生理浓度（10(-9) M）注射该激素，会抑制取食肝脏的雌性果蝇中肠的类胰蛋白酶合成，并导致血淋巴中卵黄蛋白原浓度降低以及卵母细胞生长减缓。文中讨论了Neb-TMOF在控制卵子发育中的作用以及与伊蚊TMOF的生理相似性。