Zhao Peng, Stalnaker Stephanie H, Wells Lance
Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2013;951:229-44. doi: 10.1007/978-1-62703-146-2_15.
As a complex post-translational event, the biosynthesis, structures, and functions of O-linked glycans have attracted research interests in various aspects. The recent development of novel technologies for the analysis of glycans and glycoproteins sheds new insights with regard to determining site occupancy, structure-function relationships, and the contributions of O-linked glycosylation to physiological and pathological processes. In this chapter, we refer to several approaches for the structural characterization and quantification of O-linked glycopeptides, with a focus on O-GlcNAc and O-Mannose modified glycoproteins.
作为一种复杂的翻译后修饰事件，O-连接聚糖的生物合成、结构和功能在各个方面都引起了研究兴趣。聚糖和糖蛋白分析新技术的最新进展为确定位点占有率、结构-功能关系以及O-连接糖基化对生理和病理过程的贡献提供了新的见解。在本章中，我们介绍了几种用于O-连接糖肽结构表征和定量的方法，重点是O-GlcNAc和O-甘露糖修饰的糖蛋白。
