Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA; School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Mol Cell Proteomics. 2021;20:100054. doi: 10.1074/mcp.R120.002095. Epub 2021 Feb 20.
Growing implications of glycosylation in physiological occurrences and human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by seminal methodologies and increasing capacity for detection, identification, and characterization, the past decade has provided a significant increase in the number of suitable strategies for glycan and glycopeptide quantification. Mass-spectrometry-based strategies for glycomic quantitation have grown to include metabolic incorporation of stable isotopes, deposition of mass difference and mass defect isotopic labels, and isobaric chemical labeling, providing researchers with ample tools for accurate and robust quantitation. Beyond this, workflows have been designed to harness instrument capability for label-free quantification, and numerous software packages have been developed to facilitate reliable spectrum scoring. In this review, we present and highlight the most recent advances in chemical labeling and associated techniques for glycan and glycopeptide quantification.
糖基化在生理现象和人类疾病中的意义日益重大，这促使人们集中精力通过绝对和相对定量来揭示糖组学的变化。得益于开创性的方法和不断提高的检测、鉴定和表征能力，过去十年中提供了大量适合糖和糖肽定量的策略。基于质谱的糖组定量策略已发展到包括稳定同位素的代谢掺入、质量差异和质量缺陷同位素标记的沉积以及等重化学标记，为研究人员提供了准确和稳健定量的丰富工具。除此之外，还设计了工作流程来利用仪器进行无标记定量，并且开发了许多软件包来促进可靠的谱评分。在本文中，我们介绍并强调了糖和糖肽定量的化学标记及相关技术的最新进展。
