Lavenus Julien, Lucas Mikaël, Laplaze Laurent, Guyomarc'h Soazig
Université Montpellier 2, UMR DIADE, IRD, Montpellier, France.
Methods Mol Biol. 2013;959:45-67. doi: 10.1007/978-1-62703-221-6_3.
Organogenesis is the developmental process for producing new organs from undifferentiated cells. In plants, most organs are formed during postembryonic development. Shoot lateral organs are generated in the shoot apical meristem whereas lateral roots develop outside the root apical meristem. While lateral organ formation at the shoot and root might seem quite different, recent genetic studies have highlighted numerous parallels between these processes. In particular, the dynamic accumulation of auxin has been shown to play a crucial role both as a "morphogenetic trigger" and as a morphogen in both phenomena. This suggests that a unique model system could be adopted to study organogenesis in plants. In this chapter we describe the conceptual and technical advantages that support lateral root development as a good model system for studying organogenesis in plants.
器官发生是从未分化细胞产生新器官的发育过程。在植物中，大多数器官在胚胎后发育过程中形成。茎侧器官在茎尖分生组织中产生，而侧根在根尖分生组织外部发育。虽然茎和根处的侧器官形成似乎有很大不同，但最近的遗传学研究突出了这些过程之间的许多相似之处。特别是，生长素的动态积累已被证明在这两种现象中都作为“形态发生触发因素”和形态发生素发挥关键作用。这表明可以采用一个独特的模型系统来研究植物中的器官发生。在本章中，我们描述了支持将侧根发育作为研究植物器官发生的良好模型系统的概念和技术优势。
