Chang Chungyu, Huang I-Hsiu, Hendrickx Antoni P A, Ton-That Hung
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, TX, USA.
Methods Mol Biol. 2013;966:77-95. doi: 10.1007/978-1-62703-245-2_5.
Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.
菌毛或纤毛被认为是组装在细菌表面的重要毒力决定因素。革兰氏阳性菌产生与革兰氏阴性菌不同的共价连接菌毛结构。在本章中，我们描述了三种从革兰氏阳性菌中提取、检测和观察菌毛的常用技术：（1）蛋白质免疫印迹分析，（2）免疫电子显微镜，以及（3）原子力显微镜。
