College of Quartermaster Technology, Jilin University, Changchun, PR China.
Planta Med. 2013 Jan;79(2):110-5. doi: 10.1055/s-0032-1328068. Epub 2013 Jan 8.
In this study, hemolysis, Western blot, and real-time RT-PCR assays were performed to evaluate silibinin's activity against S. aureus α-toxin secretion. In addition, live/dead cell staining and lactate dehydrogenase activity assays were introduced to examine the influence of silibinin on α-toxin-induced cell injury in human alveolar epithelial cells. Furthermore, we tested the influence of silibinin on S. aureus pneumonia in a mouse model. We show that silibinin inhibits the expression of α-toxin in a dose-dependent manner and alleviates α-toxin-induced lung cell injury. The IC50 of silibinin that inhibits the hemolytic activity of S. aureus was 14.33 µg/mL for strain 8325-4. Moreover, this compound provides effective protection on the lung injury of staphylococcal pneumonia.
在这项研究中，通过溶血试验、Western blot 分析和实时 RT-PCR 实验，评估了水飞蓟素对金黄色葡萄球菌α-毒素分泌的抑制作用。此外，还采用活/死细胞染色和乳酸脱氢酶活性检测实验，研究了水飞蓟素对α-毒素诱导的人肺泡上皮细胞损伤的影响。最后，我们在小鼠模型中测试了水飞蓟素对金黄色葡萄球菌肺炎的影响。结果表明，水飞蓟素能够以剂量依赖性方式抑制α-毒素的表达，减轻α-毒素诱导的肺细胞损伤。抑制 8325-4 株金黄色葡萄球菌溶血活性的水飞蓟素 IC50 为 14.33µg/ml。此外，该化合物对金黄色葡萄球菌肺炎的肺损伤具有有效的保护作用。
