Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
J Appl Microbiol. 2011 Dec;111(6):1551-8. doi: 10.1111/j.1365-2672.2011.05170.x. Epub 2011 Oct 31.
To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model.
Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The efficacy of chrysin against human alveolar epithelial cell injury by α-haemolysin was tested using live/dead staining or by measuring lactate dehydrogenase activity. Furthermore, we determined the protective effect of chrysin against Staph. aureus pneumonia through histopathology experiments in a mouse model. The production of α-haemolysin by Staph. aureus was inhibited when presented with an increasing subinhibitory concentration of chrysin in vitro. Consistent with this result, chrysin prevented α-haemolysin-mediated cell injury and protected mice from Staph. aureus pneumonia.
Chrysin is a potent inhibitor of α-haemolysin expression by Staph. aureus, and it conferred a significant degree of protection against Staph. aureus pneumonia.
The chrysin-mediated inhibition of α-haemolysin production and protection against Staph. aureus pneumonia may offer a new strategy in combating pathogen infections.
阐明白杨素对金黄色葡萄球菌α-溶血素产生的影响及其在肺炎小鼠模型中的保护作用。
通过溶血试验、Western blot 和实时 RT-PCR 检测白杨素对金黄色葡萄球菌α-溶血素分泌的影响。通过死活染色或测定乳酸脱氢酶活性检测白杨素对α-溶血素致肺泡上皮细胞损伤的保护作用。此外，通过小鼠肺炎模型的组织病理学实验，确定了白杨素对金黄色葡萄球菌肺炎的保护作用。体外实验中，随着亚抑菌浓度白杨素浓度的增加，金黄色葡萄球菌α-溶血素的产生受到抑制。与这一结果一致的是，白杨素可预防α-溶血素介导的细胞损伤，并保护小鼠免受金黄色葡萄球菌肺炎的侵害。
白杨素是金黄色葡萄球菌α-溶血素表达的有效抑制剂，对金黄色葡萄球菌肺炎具有显著的保护作用。
白杨素抑制α-溶血素产生并预防金黄色葡萄球菌肺炎的作用可能为抗病原体感染提供新策略。
