从海洋细菌 Vibrio sp. 中纯化和表征一种新的海藻酸盐裂解酶
Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp.
机构信息
Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, Qingdao 266003, China.
出版信息
Biotechnol Lett. 2013 May;35(5):703-8. doi: 10.1007/s10529-012-1134-x. Epub 2013 Jan 9.
UNLABELLED
An alginate lyase-producing bacterial strain, Vibrio sp. QY105, was isolated from sea mud of Qingdao. It secreted 90 % of total enzyme activity within the first 20 h of fermentation. An alginate lyase, AlyV5, with an apparent MW of 37 kDa and a specific activity of 2152 U/mg was purified from the culture supernatant. It was most active at 38 °C and pH 7.0 in 20 mM Tris/HCl. The enzyme was stable over a broad pH range (6.0-9.0) and retained ~40 % activity after holding at 90 °C for 10 min. AlyV5 showed activities towards both polyguluronate and polymannuronate, but degraded the former more efficiently. AlyV5 mainly produced disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate.
PURPOSE OF WORK
The purpose of this study is to find a polyG-preference alginate lyase for the saccharification of alginate combined with our polyM-preference alginate lyases.
未标记
从青岛海泥中分离到一株产褐藻胶裂解酶的细菌菌株，Vibrio sp. QY105。它在发酵的前 20 小时内分泌了 90%的总酶活。从培养上清液中纯化出一种褐藻胶裂解酶 AlyV5，其表观分子量为 37 kDa，比活为 2152 U/mg。该酶在 38°C 和 pH 7.0 的 20 mM Tris/HCl 中最活跃。该酶在较宽的 pH 范围（6.0-9.0）内稳定，在 90°C 下保持 10 分钟后保留约 40%的活性。AlyV5 对聚古罗糖醛酸和聚甘露糖醛酸都有活性，但对前者的降解效率更高。AlyV5 主要从聚古罗糖醛酸产生二糖、三糖和四糖，从聚甘露糖醛酸产生三糖、四糖和五糖。
工作目的
本研究的目的是找到一种对聚古罗糖醛酸盐有偏好的褐藻胶裂解酶，与我们对聚甘露糖醛酸盐有偏好的褐藻胶裂解酶结合使用，以用于褐藻酸盐的糖化。
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