Meng Cui-da, Dong Zhen, Sha Ji-chao, Li Lin, Zhu Dong-dong
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Nov;47(11):908-12.
To test the immunoglobulin free light chain (FLC) from nasal secretion(s) and serum of patients with allergic rhinitis and non-allergic rhinitis for the purpose of exploring the possible immunological mechanism.
Sixty consecutive patients were selected between September and December in 2009, involving 30 patients with allergic rhinitis and 30 patients with non-allergic rhinitis, diagnosed by symptoms, signs, SPT and sIgE. Thirty volunteers was chosen as health control (HC). ELISA was used to detect the total IgE, eosinophil cationic protein (ECP), mast cell tryptase (MCT), κFLC, λFLC in nasal secretion and serum. The data was statistically analyzed by SPSS 17.0 software.
According to the VAS scores, the nasal symptoms of AR and NAR, including sneeze, nasal discharge, nasal obstruction and nasal itching were compared. There was no statistical difference (t value was 1.189, 0.741, 0.758, 0.797, respectively, P < 0.5); In serum, κFLC, λFLC, IgE, ECP & MCT were increased in NAR compared to HC (P < 0.05); λFLC was increased in NAR compared to AR group (P < 0.05), κFLC and ECP were increased in AR. There was no significant difference between AR and NAR (P < 0.05); In nasal secretion, κFLC, λFLC, IgE, ECP and MCT were increased in AR and NAR compared to HC, and the ECP and IgE were significantly increased in AR compared to NAR (P < 0.05). ; In nasal secretion, the FLCs revealed a significantly higher correlation with MCT (r value was 0.518 and 0.484, P < 0.01), and in serum revealed a significant correlation with ECP (r value was 0.343 and 0.342, P < 0.01).
Immunoglobulin free light chain takes part in the path of physiological process of allergic rhinitis and non-allergic rhinitis with the immunological mechanism.
检测变应性鼻炎和非变应性鼻炎患者鼻分泌物及血清中的免疫球蛋白游离轻链（FLC），以探讨其可能的免疫机制。
选取2009年9月至12月连续就诊的60例患者，其中变应性鼻炎患者30例，非变应性鼻炎患者30例，均根据症状、体征、皮肤点刺试验（SPT）及特异性免疫球蛋白E（sIgE）进行诊断。选取30名志愿者作为健康对照（HC）。采用酶联免疫吸附测定（ELISA）法检测鼻分泌物及血清中的总免疫球蛋白E（IgE）、嗜酸性粒细胞阳离子蛋白（ECP）、肥大细胞类胰蛋白酶（MCT）、κFLC、λFLC。数据采用SPSS 17.0软件进行统计学分析。
根据视觉模拟评分（VAS）对变应性鼻炎（AR）和非变应性鼻炎（NAR）的鼻症状，包括喷嚏、流涕、鼻塞及鼻痒进行比较。差异无统计学意义（t值分别为1.189、0.741、0.758、0.797，P<0.5）；血清中，与HC相比，NAR组的κFLC、λFLC、IgE、ECP及MCT升高（P<0.05）；与AR组相比，NAR组的λFLC升高（P<0.05），AR组的κFLC及ECP升高。AR与NAR之间差异无统计学意义（P<0.05）；鼻分泌物中，与HC相比，AR和NAR组的κFLC、λFLC、IgE、ECP及MCT升高，与NAR组相比，AR组的ECP及IgE显著升高（P<0.05）；鼻分泌物中，FLC与MCT的相关性显著更高（r值分别为0.518和0.484，P<0.01），血清中与ECP的相关性显著（r值分别为0.343和0.342，P<0.01）。
免疫球蛋白游离轻链通过免疫机制参与变应性鼻炎和非变应性鼻炎的生理病理过程。
