Faculty of Biology, Laboratory of Histology and Developmental Biology, University of Bucharest, Splaiul Independentei 91-95, R-050095, Bucharest, Romania.
Microsc Microanal. 2013 Feb;19(1):233-42. doi: 10.1017/S1431927612013797. Epub 2013 Jan 10.
Dermal bone is an important component of the teleost fins, and its ability to regenerate after fin amputation appears to be unlimited. The organic bone matrix contain type I collagen fibers, proteoglycans enriched in chondroitin sulfate, and noncollagenous matrix protein such as osteocalcin, osteopontin, and osteonectin. These molecules are synthesized by fin osteoblasts. Inorganic components chiefly consist of calcium and phosphate that form crystals of hydroxyapatite. Fin rays are described as models to study ossification. Due to this, the identification of the components involved in the synthesis of the organic and inorganic components of lepidotrichial bone are of great interest for the analysis of skeletal disorders in fish ossification. The present study investigates expression of alkaline phosphatase, osteopontin, osteocalcin, and chondroitin sulfate during pectoral fin regeneration in Carassius auratus gibelio. Alkaline phosphatase reaction has been found in the epidermis covering the wound, proximal blastema, near the cells that surround newly-formed lepidotrichia matrix and the tips of regenerating fin rays. Osteopontin has been observed throughout the regeneration blastema but excluded from the scleroblasts lining the inner side of the lepidotrichia. Osteocalcin and chondroitin sulfate expression coincides with the onset of mineralization of lepidotrichial matrix, suggesting its involvement in bone mineralization.
真皮骨是硬骨鱼鳍的重要组成部分，其在鳍截肢后再生的能力似乎是无限的。有机骨基质包含 I 型胶原纤维、富含硫酸软骨素的蛋白聚糖，以及非胶原蛋白基质蛋白，如骨钙素、骨桥蛋白和骨连蛋白。这些分子由鳍成骨细胞合成。无机成分主要由钙和磷组成，形成羟基磷灰石晶体。鳍射线被描述为研究骨化的模型。因此，鉴定参与真皮骨有机和无机成分合成的成分对于分析鱼类骨化中的骨骼疾病具有重要意义。本研究调查了碱性磷酸酶、骨桥蛋白、骨钙素和硫酸软骨素在 Carassius auratus gibelio 胸鳍再生过程中的表达。碱性磷酸酶反应在覆盖伤口的表皮、近端芽基、新形成的真皮基质周围的细胞以及再生鳍射线的尖端中被发现。骨桥蛋白在整个再生芽基中被观察到，但被排列在真皮内部的成骨细胞排除在外。骨钙素和硫酸软骨素的表达与真皮基质矿化的开始同时发生，表明其参与了骨矿化。
