[宫腔镜下宫腔粘连切除术前后宫腔液中黏附相关细胞因子的表达]

[Expression of adhesion-related cytokines in the uterine fluid after transcervical resection of adhesion].

作者信息

Tao Zhi, Duan Hua

机构信息

Centre of Minimally Invasive Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, China.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 2012 Oct;47(10):734-7.

PMID:23302729

Abstract

OBJECTIVE

To study the concentration of adhesion-related cytokines, transforming growth factor (TGF) β(1), platelet-derived growth factor (PDGF) BB, b-fibroblast growth factor (bFGF) in intrauterine fluid and their roles in the intrauterine-adhesion reformation.

METHODS

From August 2009 to February 2010, 18 patients with moderate or severe intrauterine adhesions underwent transcervical resection of intrauterine adhesion as study group, in the mean time, 20 patients with incomplete septate uterus underwent transcervical resection of septum as control group. The charge of each patient's intrauterine fluid was collected at the time of 3, 6, 9, 12, 24, 48 and 72 hours, respectively, then the concentration of the three cytokines was measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS

The concentration of the expression of TGF-β(1) in the intrauterine fluid at 3, 6, 9, 12 hours after operation in study group was (3.6 ± 0.9), (10.4 ± 1.1), (7.6 ± 1.2), (7.2 ± 1.3) ng/ml, respectively; PDGF-BB was (2.6 ± 0.6), (3.5 ± 0.5), (5.4 ± 1.0), (5.7 ± 0.8) ng/ml, respectively; bFGF was (16.9 ± 1.3), (95.8 ± 17.8), (330.9 ± 70.5), (1303.3 ± 117.4) ng/ml, respectively. The expression of TGF-β(1) in control group was (3.0 ± 0.6), (7.5 ± 0.6), (5.4 ± 0.6), (4.6 ± 0.8) ng/ml, respectively; PDGF-BB was (2.5 ± 0.4), (2.6 ± 0.5), (4.7 ± 0.6), (4.4 ± 0.4) ng/ml, respectively; bFGF was (19.1 ± 2.4), (82.9 ± 21.8), (249.0 ± 54.2), (775.6 ± 178.8) ng/ml, respectively. The concentration of TGF-β(1), PDGF-BB and bFGF reached the peak during the time of 3 - 24 hours, 6 - 48 hours, 6 - 72 hours after surgery, respectively. The concentration of TGF-β(1), PDGF-BB and bFGF in study group were significantly higher than that in control group (P < 0.05).

CONCLUSIONS

Concentration of TGF-β(1), PDGF-BB and bFGF was positively associated with area of transcervical resection of intrauterine adhesion, those cytokines might be involved in reformatin of intrauterine-adhesion.

摘要

目的

研究宫腔液中黏附相关细胞因子转化生长因子（TGF）β(1)、血小板衍生生长因子（PDGF）BB、碱性成纤维细胞生长因子（bFGF）的浓度及其在宫腔粘连重塑中的作用。

方法

2009年8月至2010年2月，18例中重度宫腔粘连患者行宫腔镜下宫腔粘连切除术作为研究组，同时选取20例不全纵隔子宫患者行宫腔镜下纵隔切除术作为对照组。分别于术后3、6、9、12、24、48和72小时收集每位患者的宫腔液，然后采用酶联免疫吸附测定（ELISA）法检测三种细胞因子的浓度。

结果

研究组术后3、6、9、12小时宫腔液中TGF-β(1)的表达浓度分别为（3.6±0.9）、（10.4±1.1）、（7.6±1.2）、（7.2±1.3）ng/ml；PDGF-BB分别为（2.6±0.6）、（3.5±0.5）、（5.4±1.0）、（5.7±0.8）ng/ml；bFGF分别为（1,6.9±1.3）、（95.8±17.8）、（330.9±70.5）、（1303.3±117.4）ng/ml。对照组TGF-β(1)的表达浓度分别为（3.0±0.6）、（7.5±0.6）、（5.4±0.6）、（4.6±0.8）ng/ml；PDGF-BB分别为（2.5±0.4）、（2.6±0.5）、（4. 7±0.6）、（4.4±0.4）ng/ml；bFGF分别为（19.1±2.4）、（82.9±21.8）、（249.0±54.2）、（775.6±178.8）ng/ml。TGF-β(1)、PDGF-BB和bFGF的浓度分别在术后3 - 24小时内、6 - 48小时内、6 - 72小时内达到峰值。研究组TGF-β(1)、PDGF-BB和bFGF的浓度显著高于对照组（P<0.05）。