Youssef Samar Samir, Nasr Aml S, El Zanaty Taher, El Rawi Rasha Sayed, Mattar Mervat M
Microbial Biotechnology Department, National Research Centre, Cairo 12311, Egypt.
Hepat Res Treat. 2012;2012:429784. doi: 10.1155/2012/429784. Epub 2012 Dec 12.
Background. Occult hepatitis C virus infection (OCI) was identified as a new form of Hepatitis C virus (HCV), characterized by undetectable HCV antibodies and HCV RNA in serum, while HCV RNA is detectable in liver and peripheral blood cells only. Aim. The aim of this study was to investigate the occurrence of OCI in Egyptian patients with lymphoproliferative disorders (LPDs) and to compare its prevalence with that of HCV in those patients. Subjects and Methods. The current study included 100 subjects, 50 of them were newly diagnosed cases having different lymphoproliferative disorders (patients group), and 50 were apparently healthy volunteers (controls group). HCV antibodies were detected by ELISA, HCV RNA was detected in serum and peripheral blood mononuclear cells (PBMCs) by reverse transcription polymerase chain reaction(RT-PCR), and HCV genotype was detected by INNO-LiPA. Results. OCI was detected in 20% of patients group, compared to only 4% OCI in controls group. HCV was detected in 26% of patients group with a slightly higher prevalence. There was a male predominance in both HCV and OCI. All HCV positive patients were genotype 4. Conclusion. Our data revealed occurrence of occult HCV infection in Egyptian LPD patients at a prevalence of 20% compared to 26% of HCV.
背景。隐匿性丙型肝炎病毒感染（OCI）被确认为丙型肝炎病毒（HCV）的一种新形式，其特征是血清中检测不到HCV抗体和HCV RNA，而仅在肝脏和外周血细胞中可检测到HCV RNA。目的。本研究的目的是调查埃及淋巴增殖性疾病（LPD）患者中OCI的发生情况，并将其患病率与这些患者中HCV的患病率进行比较。对象与方法。本研究纳入100名受试者，其中50名是新诊断的患有不同淋巴增殖性疾病的病例（患者组），50名是明显健康的志愿者（对照组）。通过酶联免疫吸附测定（ELISA）检测HCV抗体，通过逆转录聚合酶链反应（RT-PCR）检测血清和外周血单个核细胞（PBMC）中的HCV RNA，并通过INNO-LiPA检测HCV基因型。结果。患者组中20%检测到OCI，而对照组中仅4%检测到OCI。患者组中26%检测到HCV，患病率略高。HCV和OCI在男性中均占主导。所有HCV阳性患者均为4型基因型。结论。我们的数据显示，埃及LPD患者中隐匿性HCV感染的发生率为20%，而HCV的发生率为26%。
