Almogren A, Shakoor Z, Hamam K D
Department of Pathology, College of Medicine and University Hospitals King Saud University, Riyadh 11461, Saudi Arabia.
Br J Biomed Sci. 2012;69(4):159-63.
Most patients with chronic renal failure develop end-stage renal disease (ESRD) that requires renal transplantation. This study investigates the possible associations between human leucocyte antigen (HLA) Class I and Class II molecules with ESRD. Genotyping data (HLA) obtained between 2005 and 2009 on 235 unrelated Saudi patients (147 males, 88 females; mean age: 58 +/- 7 years) with ESRD awaiting renal transplantation were assessed retrospectively at the King Khalid University Hospital. Data were compared with the results on 60 normal, healthy, unrelated Saudi individuals (37 males and 23 females; mean age: 51 +/- 5 years). HLA Class I and Class II antigens were detected by lymphocytotoxicity and a polymerase chain reaction (PCR) method using DNA sequence-specific primers. Although present in small numbers, HLA Cw2 was found in significantly fewer patients (n = 11; 4.68%) compared to normal subjects (n = 9; 15%) and was found to confer protection against ESRD (P = 0.005; relative risk [RR]: 3.594, 95% confidence interval [CI]: 1.415-9.126). Among the HLA Class II antigens, HLA DQB103(8) was detected more frequently in the patient group (n = 65; 27.6%) than in the normal controls (n = 9; 15%) and was positively associated with risk of ESRD (P = 0.04; RR: 0.462, 95% CI: 0.215-0.991). No significant differences were observed between the two groups in respect of HLA-A2, HLA-B50(21), HLA-B51(5) and HLA-Cw7 (HLA Class I), and HLA-DRB104, HLA-DRB107 and HLA-DQB102 (HLA Class II). Occurrence of the most frequent HLA alleles was no different between the ESRD group and the controls. The protective role of HLA-Cw2 and the marginal susceptibility associated with HLA-DQBI*03(8) for ESRD requires further investigation.
大多数慢性肾衰竭患者会发展为终末期肾病（ESRD），需要进行肾移植。本研究调查了人类白细胞抗原（HLA）I类和II类分子与ESRD之间可能存在的关联。在哈利德国王大学医院对2005年至2009年间获得的235例等待肾移植的沙特ESRD无关患者（147例男性，88例女性；平均年龄：58±7岁）的基因分型数据（HLA）进行了回顾性评估，并与60例正常、健康、无关的沙特个体（37例男性和23例女性；平均年龄：51±5岁）的结果进行了比较。采用淋巴细胞毒性和聚合酶链反应（PCR）方法，使用DNA序列特异性引物检测HLA I类和II类抗原。尽管数量较少，但与正常受试者（n = 9；15%）相比，HLA Cw2在患者中的发现显著较少（n = 11；4.68%），且发现其对ESRD具有保护作用（P = 0.005；相对风险[RR]：3.594，95%置信区间[CI]：1.415 - 9.126）。在HLA II类抗原中，HLA DQB103(8)在患者组（n = 65；27.6%）中的检测频率高于正常对照组（n = 9；15%），且与ESRD风险呈正相关（P = 0.04；RR：0.462，95% CI：0.215 - 0.991）。在HLA - A2、HLA - B50(21)、HLA - B51(5)和HLA - Cw7（HLA I类）以及HLA - DRB104、HLA - DRB107和HLA - DQB102（HLA II类）方面，两组之间未观察到显著差异。ESRD组和对照组之间最常见的HLA等位基因出现情况没有差异。HLA - Cw2的保护作用以及HLA - DQBI*03(8)与ESRD相关的边缘易感性需要进一步研究。
