Kapitola J, Jahoda I, Stĕpánková M
Laborator pro endokrinlogii a metabolismus a III. interní klinika fakulty vseobecného lékarství Univerzity Karlovy, Praha.
Sb Lek. 1990 Jan;92(1):6-11.
In previous experiments (4) we observed in laboratory rats after a low Ca content diet with no vitamin D a decrease of blood flow through bones. In this study we follow whether the effect of the deficit diet is influenced by age or sex of experimental animals. Tibia blood flow in rats is established by 85Sr-microparticles, bone density is estimated by weighing the bone under water. After 10 days of feeding by the deficit diet 85Sr-microparticles catchment in young males is decreased to 74% of that of controls, in young females to 61%, blood flow is decreased to 46% and 51% respectively. In older males after the diet the microparticles catchment is significantly decreased to 63%, blood flow is non-significantly decreased to 82% (as a result of higher cardiac output). In the category of older females the effect of deficient diet is not demonstrable as regards both microparticles catchment and blood flow. Bone density in preliminary experiment after 40 days of diet is significantly decreased in males of both age categories and in young females, in the main experiment after 10 days of diet it is decreased only in the categories of young males and females. From the results obtained in following the effect of diet deficient in Ca and vitamin D on density and local blood flow in tibia of rats can be concluded 1) that in this respect younger rats are more susceptible, 2) in the category of older males the effect is partly demonstrable in males, but not in females.
在之前的实验（4）中，我们观察到给实验大鼠喂食低钙且无维生素D的饮食后，其骨骼的血流量减少。在本研究中，我们探究了这种缺乏饮食的影响是否受实验动物年龄或性别的影响。通过85锶微粒测定大鼠胫骨的血流量，通过在水下称量骨骼来估计骨密度。在喂食缺乏饮食10天后，年轻雄性大鼠对85锶微粒的摄取量降至对照组的74%，年轻雌性大鼠降至61%，血流量分别降至46%和51%。在老年雄性大鼠中，饮食后微粒摄取量显著降至63%，血流量非显著降至82%（由于心输出量较高）。在老年雌性大鼠中，无论是微粒摄取还是血流量方面，缺乏饮食的影响均未得到证实。在初步实验中，饮食40天后，两个年龄组的雄性大鼠和年轻雌性大鼠的骨密度均显著降低；在主要实验中，饮食10天后，仅年轻雄性和雌性大鼠的骨密度降低。从观察钙和维生素D缺乏饮食对大鼠胫骨密度和局部血流量影响的结果可以得出：1）在这方面，年轻大鼠更易受影响；2）在老年雄性大鼠中，这种影响在雄性中部分得到证实，但在雌性中未得到证实。
