Laboratory for Biomedical Microtechnology, Department of Microsystems Engineering-IMTEK and the Bernstein Center Freiburg, University of Freiburg, Freiburg, Germany.
Lab Chip. 2013 Feb 21;13(4):579-88. doi: 10.1039/c2lc40874k.
In optogenetics, neurons are genetically modified to become sensitive to light and thus, they can be stimulated or inhibited by light of certain wavelengths. In this work, we describe the fabrication of a polymer-based shaft electrode as a tool for optogenetics. This device can conduct light as well as fluids to a target brain region and record electrical neural signals from the same part of the tissue simultaneously. It is intended to facilitate optogenetic in vivo experiments with those novel multimodal neural probes or polymer optrodes. We used microsystems technology to integrate an SU-8 based waveguide and fluidic channel into a polyimide-based electrode shaft to allow simultaneous optical stimulation, fluid delivery, and electrophysiological recording in awake behaving animals. In a first acute proof-of-concept experiment in genetically modified mice, our device recorded single unit activity that was modulated by laser light transmitted into the tissue via the integrated waveguide.
在光遗传学中，通过基因改造使神经元对光敏感，从而可以用光的特定波长来刺激或抑制神经元。在这项工作中，我们描述了一种基于聚合物的轴电极的制造，作为光遗传学的工具。该器件可以将光以及流体传输到目标脑区，并同时从组织的同一部分记录电神经信号。它旨在促进具有新型多模态神经探针或聚合物光极的活体光遗传学实验。我们使用微系统技术将基于 SU-8 的波导和微流道集成到聚酰亚胺基电极轴中，以允许在清醒的行为动物中进行同时的光刺激、流体输送和电生理记录。在首例经基因改造的小鼠的急性概念验证实验中，我们的器件通过集成波导传输到组织中的激光记录了由激光调制的单个单元活动。
