Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil.
Appl Biochem Biotechnol. 2013 Feb;169(4):1373-85. doi: 10.1007/s12010-012-0053-6. Epub 2013 Jan 11.
Trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. Best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 U mL(-1) for xylanase, 1.9 U mL(-1) for endoglucanase, 0.25 U mL(-1) for FPase, and 0.17 U mL(-1) for β-glucosidase) after 3-4 days fermentation. The maximal enzymatic activity for endoglucanase, FPase, and xylanase were observed at 50-60 °C and pH 4.0-5.0, whereas thermal stability at 50 °C (CMCase and FPase) or 40 °C (xylanase) was obtained after 8 h. Zymograms have shown two bands of 104 and 200 kDa for endoglucanases and three bands for xylanase (23, 36, and 55.7 kDa). The results obtained with T. atroviride strain 676 were comparable to those obtained with the cellulolytic strain Trichoderma reesei RUT-C30, indicating, in the studied conditions, its great potential for biotechnological application, especially lignocellulose biomass hydrolysis.
研究了深绿木霉 676 菌株利用木质纤维素残余生物质生产一些木质纤维素酶的效率。当使用未处理的 3.0%（w/v）甘蔗渣时，获得了最佳结果（木聚糖酶为 61.3 U mL(-1)，内切葡聚糖酶为 1.9 U mL(-1)，纤维二糖磷酸酶为 0.25 U mL(-1)，β-葡萄糖苷酶为 0.17 U mL(-1)），发酵 3-4 天后。内切葡聚糖酶、纤维二糖磷酸酶和木聚糖酶的最大酶活在 50-60°C 和 pH 4.0-5.0 时观察到，而在 50°C（CMCase 和 FPase）或 40°C（木聚糖酶）下经过 8 小时后获得了热稳定性。酶谱显示内切葡聚糖酶有两条 104 和 200 kDa 的带，木聚糖酶有三条带（23、36 和 55.7 kDa）。深绿木霉 676 菌株获得的结果与纤维素酶菌株里氏木霉 RUT-C30 获得的结果相当，表明在研究条件下，其在生物技术应用，特别是木质纤维素生物质水解方面具有巨大的潜力。
