de Almeida Maíra N, Guimarães Valéria M, Falkoski Daniel L, Paes Guilherme B T, Ribeiro José Ivo, Visser Evan M, Alfenas Rafael F, Pereira Olinto L, de Rezende Sebastião T
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil.
Appl Biochem Biotechnol. 2014 Feb;172(3):1332-46. doi: 10.1007/s12010-013-0572-9. Epub 2013 Oct 30.
Enzymatic hydrolysis is an important but expensive step in the production of ethanol from biomass. Thus, the production of efficient enzymatic cocktails is of great interest for this biotechnological application. The production of endoglucanase and xylanase activites from F. verticillioides were optimized in a factorial design (2(5)) followed by a CCDR design. Endoglucanase and xylanase activities increased from 2.8 to 8.0 U/mL and from 13.4 to 114 U/mL, respectively. The optimal pH and temperature were determined for endoglucanase (5.6, 80 °C), cellobiase (5.6, 60 °C), FPase (6.0, 55 °C) and xylanase (7.0, 50 °C). The optimized crude extract was applied in saccharification and fermentation of sugarcane bagasse from which 9.7 g/L of ethanol was produced at an ethanol/biomass yield of 0.19.
酶水解是生物质生产乙醇过程中的一个重要但昂贵的步骤。因此，生产高效的酶混合物对于这种生物技术应用具有极大的吸引力。在析因设计（2(5)）之后采用CCDR设计，对来自轮枝镰孢菌的内切葡聚糖酶和木聚糖酶活性的生产进行了优化。内切葡聚糖酶和木聚糖酶活性分别从2.8 U/mL增加到8.0 U/mL和从13.4 U/mL增加到114 U/mL。确定了内切葡聚糖酶（5.6，80℃）、纤维二糖酶（5.6，60℃）、滤纸酶（6.0，55℃）和木聚糖酶（7.0，50℃）的最佳pH值和温度。将优化后的粗提取物应用于甘蔗渣的糖化和发酵，从中以0.19的乙醇/生物质产率生产出9.7 g/L的乙醇。
