Department of Food and Nutritional Sciences, The University of Reading, Whiteknights, PO Box 226, Reading, RG6 6AP, UK,
Eur J Nutr. 2013 Dec;52(8):1853-63. doi: 10.1007/s00394-012-0487-1. Epub 2013 Jan 10.
There is growing evidence that probiotics confer health benefits to the host by modulating immune function, especially in older people, where immunosenescence is a feature even of healthy ageing. The aim of this study was to investigate the effect of a probiotic drink containing Lactobacillus casei Shirota (LcS) on immune function in a healthy non-immunocompromised older population.
Thirty healthy old volunteers were recruited into a randomized placebo-controlled, single-blind crossover study. The volunteers were supplemented with the probiotic drink containing 1.3 × 10(10) CFU LcS or skimmed milk per day for 4 weeks, followed by 4 weeks of washout and were crossed over to the other treatment. Peripheral blood and saliva samples were collected at baseline and end of each treatment.
Probiotic consumption was associated with a significant increase in natural killer (NK) cell activity relative to baseline and a significant decrease in the mean fluorescence intensity of CD25 expression in the resting T cells compared with placebo. Additionally, there was a trend towards an increased ratio of IL-10 to IL-12 relative to baseline after LcS intake.
Consumption of a probiotic drink containing LcS improved NK cell activity and tended to produce a more anti-inflammatory cytokine profile in an older population.
越来越多的证据表明益生菌通过调节免疫功能为宿主带来健康益处，尤其是在老年人中，免疫衰老甚至是健康衰老的特征。本研究旨在调查含有干酪乳杆菌 Shirota（LcS）的益生菌饮料对健康、非免疫受损的老年人群免疫功能的影响。
30 名健康的老年志愿者被招募到一项随机、安慰剂对照、单盲交叉研究中。志愿者每天补充含有 1.3×10(10)CFU LcS 或脱脂牛奶的益生菌饮料，持续 4 周，然后进行 4 周的洗脱期，并交叉到另一种治疗。在基线和每个治疗结束时采集外周血和唾液样本。
与基线相比，益生菌摄入与自然杀伤（NK）细胞活性的显著增加相关，与安慰剂相比，静止 T 细胞中 CD25 表达的平均荧光强度显著降低。此外，与基线相比，LcS 摄入后，IL-10 与 IL-12 的比值有增加的趋势。
含有 LcS 的益生菌饮料的摄入可提高 NK 细胞活性，并在老年人群中倾向于产生更抗炎的细胞因子谱。
