Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka, 537-0025, Japan,
J Nat Med. 2013 Oct;67(4):838-43. doi: 10.1007/s11418-012-0739-7. Epub 2013 Jan 10.
The official Japanese method for analyzing aristolochic acid I (AA-I) in Asiasarum root using conventional high-performance liquid chromatography (HPLC) is described in the Japanese Pharmacopoeia, Sixteenth Edition. Interfering peaks of AA-I sometimes appear after HPLC analysis of crude drugs. A selective analytical method is needed to determine definitively whether AA-I is present in crude drugs. In this study, we developed a selective method that combined solid-phase extraction and liquid chromatography/mass spectrometry (LC/MS) which may be useful for identifying AA-I in crude drugs and for quality control.
日本《第十六版日本药局方》中描述了用常规高效液相色谱法（HPLC）分析细辛中马兜铃酸 I（AA-I）的官方方法。在对生药进行 HPLC 分析后，AA-I 的干扰峰有时会出现。因此，需要一种选择性的分析方法来确定生药中是否存在 AA-I。在这项研究中，我们开发了一种结合固相萃取和液相色谱/质谱（LC/MS）的选择性方法，该方法可能有助于鉴定生药中的 AA-I，并进行质量控制。
