State Key Laboratory of Oncology in South China, Department of Radiation Oncology, Sun Yat-sen University Cancer Center, 651 Dongfeng Road East, Guangzhou 510060, People's Republic of China.
Med Oncol. 2013 Mar;30(1):360. doi: 10.1007/s12032-012-0360-3. Epub 2013 Jan 10.
Dicer11 plays an important role in generation of microRNA and is dysregulated in a variety of human cancers. The purpose of this study was to evaluate Dicer11 expression and its prognostic value in nasopharyngeal carcinoma (NPC). The protein expression of Dicer1 was examined by immunohistochemistry in 276 NPC specimens, and the mRNA levels of Dicer1 were analyzed by qRT-PCR in 56 NPC and 11 nasopharyngitis tissues. Cox regression analysis was used to identify independent prognostic factors, and a prognostic score model was constructed for survival prediction. Expression of Dicer1 was downregulated in NPC tissues at both the mRNA and the protein levels, and there was a notable positive correlation between the expression levels of Dicer1 mRNA and protein. Low Dicer1 expression was positively correlated with distant metastasis (P < 0.01) and death (P = 0.01). In addition, low expression of Dicer1 was significantly associated with poorer overall survival (HR, 2.32; 95 % CI 1.30-4.14; P < 0.01) and poorer distant metastasis-free survival (HR, 2.56; 95 % CI 1.39-4.74; P < 0.01). Furthermore, multivariate analysis showed that low expression of Dicer1 and tumor-node-metastasis (TNM) stage was independent prognostic indicators for NPC patients. A prognostic score model combining the Dicer1 expression and TNM stage had a better prognostic value than the TNM stage alone model or Dicer1 expression alone model (P < 0.05). Dicer1 was downregulated in NPC tissues at both the mRNA and the protein levels, and low expression of Dicer1 could be served as novel prognostic biomarker for NPC patients.
Dicer11 在 microRNA 的生成中发挥重要作用，并且在多种人类癌症中失调。本研究旨在评估 Dicer11 在鼻咽癌（NPC）中的表达及其预后价值。通过免疫组织化学法检测 276 例 NPC 标本中 Dicer11 的蛋白表达，通过 qRT-PCR 分析 56 例 NPC 和 11 例鼻咽炎症组织中 Dicer1 的 mRNA 水平。Cox 回归分析用于确定独立的预后因素，并构建用于生存预测的预后评分模型。Dicer1 的表达在 NPC 组织中在 mRNA 和蛋白水平上均下调，并且 Dicer1 mRNA 和蛋白的表达水平之间存在显著的正相关。Dicer1 低表达与远处转移（P < 0.01）和死亡（P = 0.01）呈正相关。此外，Dicer1 低表达与总生存期较差（HR，2.32；95%CI，1.30-4.14；P < 0.01）和远处无转移生存期较差显著相关（HR，2.56；95%CI，1.39-4.74；P < 0.01）。此外，多变量分析表明，Dicer1 低表达和肿瘤-淋巴结-转移（TNM）分期是 NPC 患者的独立预后指标。结合 Dicer1 表达和 TNM 分期的预后评分模型比单独的 TNM 分期模型或 Dicer1 表达模型具有更好的预后价值（P < 0.05）。Dicer1 在 NPC 组织中在 mRNA 和蛋白水平上均下调，并且 Dicer1 的低表达可以作为 NPC 患者的新型预后生物标志物。
