Scialfa Exequiel, Brihuega Bibiana, Venzano Agustín, Morris Winston Eduardo, Bolpe Jorge, Schettino Mateo
División Zoonosis Rurales, España 770, Azul (7300), Provincia de Buenos Aires, Argentina.
J Wildl Dis. 2013 Jan;49(1):168-72. doi: 10.7589/2011-07-219.
To identify carriers of Leptospira spp. in Argentina, wild animals were trapped in Buenos Aires Province during three nights, capturing 12 Didelphis albiventris (white-eared opossum), six Chaetophractus villosus (big hairy armadillo), five Lycalopex griseus (South American gray fox), and two Conepatus chinga (Molina's hog-nosed skunk). All were tested by microscopic agglutination test, and five (two gray foxes, two armadillos, and one skunk) were positive for Leptospira interrogans serovars Canicola and Icterohaemorrhagiae, L. borgpetersenii serovar Castellonis, and L. kirschneri serovar Grippotyphosa, at titers of 1:50 and 1:100. Kidney tissue from all animals was cultured, and one isolate of L. interrogans from a gray fox was obtained. Hamsters inoculated with the isolate died after 6 days with no macroscopic lesions at necropsy. However, histologic examination revealed glomerulonephritis, interstitial nephritis, and pneumonia. The Leptospira strain from the South American gray fox was analyzed serologically and its pathogenicity was established. Genotyping through multiple-locus variable-number tandem repeat analysis showed that the strain was a new genotype related to the L. interrogans serogroup Icterohaemorrhagiae.
为了在阿根廷识别钩端螺旋体属的携带者，在布宜诺斯艾利斯省的三个夜晚捕获了野生动物，捕获了12只白耳负鼠（Didelphis albiventris）、6只大毛犰狳（Chaetophractus villosus）、5只南美灰狐（Lycalopex griseus）和2只莫利纳猪鼻臭鼬（Conepatus chinga）。所有动物都通过显微镜凝集试验进行检测，5只（2只灰狐、2只犰狳和1只臭鼬）对问号钩端螺旋体血清型犬型和出血性黄疸型、波摩那钩端螺旋体血清型 Castellonis以及克氏钩端螺旋体血清型Grippotyphosa呈阳性，滴度为1:50和1:100。对所有动物的肾脏组织进行培养，从一只灰狐中获得了一株问号钩端螺旋体分离株。接种该分离株的仓鼠在6天后死亡，尸检时无宏观病变。然而，组织学检查显示有肾小球肾炎、间质性肾炎和肺炎。对来自南美灰狐的钩端螺旋体菌株进行了血清学分析并确定了其致病性。通过多位点可变数目串联重复分析进行基因分型表明，该菌株是一种与问号钩端螺旋体血清群出血性黄疸型相关的新基因型。
