Kin Marta S, Brihuega Bibiana, Fort Marcelo, Delgado Fernando, Bedotti Daniel, Casanave Emma B
Chordate Biology, Department of Natural and Exact Sciences, UNLPam, Santa Rosa, La Pampa, Argentina.
Institute of Pathobiology, CICVyA - CNIA, INTA, Morón, Buenos Aires, Argentina.
Rev Argent Microbiol. 2015 Jan-Mar;47(1):41-6. doi: 10.1016/j.ram.2015.01.005. Epub 2015 Mar 6.
Leptospirosis is a zoonosis of worldwide distribution. The aim of this study was to examine the presence of antibodies against 21 Leptospira reactive serovars in Chaetophractus villosus in La Pampa province, Argentina, using the microscopic agglutination test (MAT). Pathologic changes compatible with leptospirosis and in situ detection of the agent by immunohistochemistry were studied in 24 and 3 individuals respectively. Only 35/150 (23.3%) serum samples had antibodies against Leptospira sp. Six percent of the samples reacted with serovar Canicola, 4.7% with serovar Castellonis, 1.3% with serovar Icterohemorrhagieae and 0.7% with serovar Hardjo. Sixteen (10.6%) serum samples agglutinated with Castellonis-Icterohemorrhagiae and Canicola-Castellonis serovars, both with 4.7%, and Canicola-Hardjo and Castellonis-Canicola-Icterohemorrhagiae both with 0.6%. Fourteen animals had variable degrees of lesions, which were more severe in animals with higher serological titers (3200), and Leptospira sp. was detected in 3 animals by immunohistochemistry. These results represent the first record of the presence of Leptospira in C. villosus in La Pampa.
钩端螺旋体病是一种分布于全球的人畜共患病。本研究旨在采用显微镜凝集试验（MAT）检测阿根廷潘帕斯省绒毛犰狳体内针对21种钩端螺旋体反应性血清型的抗体。分别对24只和3只动物研究了与钩端螺旋体病相符的病理变化以及通过免疫组织化学对病原体进行原位检测。仅35/150（23.3%）份血清样本含有抗钩端螺旋体属的抗体。6%的样本与犬型血清型反应，4. /150（23.3%）份血清样本含有抗钩端螺旋体属的抗体。6%的样本与犬型血清型反应，4.7%与 Castellonis 血清型反应，1.3%与出血性黄疸型血清型反应，0.7%与哈德乔血清型反应。16份（10.6%）血清样本与 Castellonis - 出血性黄疸型及犬型 - Castellonis 血清型凝集，二者均为4.7%，犬型 - 哈德乔及 Castellonis - 犬型 - 出血性黄疸型均为0.6%。14只动物有不同程度的病变，血清学滴度较高（3200）的动物病变更严重，通过免疫组织化学在3只动物中检测到钩端螺旋体属。这些结果代表了潘帕斯省绒毛犰狳体内钩端螺旋体存在情况的首次记录。 （注：原文中“4. /150”表述有误，应为“4.7%/150”，译文暂按原文翻译）
