一项关于重组 FSH-LH 卵巢刺激后人类生育人群中减数分裂分离的研究。
A study of meiotic segregation in a fertile human population following ovarian stimulation with recombinant FSH-LH.
机构信息
Institut Marquès, C/Manuel Girona no. 33 La Masia-Clínica CIMA, Barcelona, Spain.
出版信息
J Assist Reprod Genet. 2013 Feb;30(2):269-74. doi: 10.1007/s10815-012-9905-9. Epub 2013 Jan 10.
OBJECTIVE
The aim of the study is to investigate the meiotic segregation in fresh eggs from anonymous egg donors and to analyze the baseline levels of aneuploidy in this population.
RESULTS
The study includes the largest series of donor eggs so far studied: 203 eggs from donors aged between 20 and 31 years. No diagnosis was obtained in 10.8 % of cases (22/ 203). The biopsy of the first and second polar bodies was completed in a sequential manner on day 0 and day 1 of embryo development. Chromosomes 13, 16, 18, 21 and 22 are analyzed by means of the FISH test. The diagnosable fertilized eggs gave an aneuploidy rate of 19.1 % (31/162), with 83.8 % (26/31) of the errors produced during meiosis I, 12.9 % (4/31) produced during meiosis II, and 3.2 % (1/31) produced during both meiosis I and II. The premature division of sister chromatids is the main source of meiotic error during Meiosis I, resulting in the creation of oocyte aneuploidy.
CONCLUSIONS
FISH analysis of the first and second polar body in donor oocytes gave an aneuploidy rate of 19.1 %. This study shows the majority of errors occur during Meiosis I.
目的
本研究旨在研究匿名供卵者新鲜卵母细胞的减数分裂分离，并分析该人群的基础非整倍体水平。
结果
本研究纳入了迄今为止最大的供卵者卵母细胞系列：203 枚年龄在 20 至 31 岁之间的供卵者的卵母细胞。10.8%（22/203）的病例未获得诊断。第 0 天和第 1 天胚胎发育时，按顺序完成第一极体和第二极体的活检。通过荧光原位杂交（FISH）试验分析 13、16、18、21 和 22 号染色体。可诊断的受精卵的非整倍体率为 19.1%（31/162），其中 83.8%（26/31）的错误发生在减数分裂 I 期，12.9%（4/31）发生在减数分裂 II 期，3.2%（1/31）发生在减数分裂 I 和 II 期。姐妹染色单体的过早分离是减数分裂 I 期减数分裂错误的主要来源，导致卵母细胞非整倍体的产生。
结论
供体卵母细胞第一极体和第二极体的 FISH 分析显示非整倍体率为 19.1%。本研究表明，大多数错误发生在减数分裂 I 期。
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