Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria.
Invest Ophthalmol Vis Sci. 2013 Feb 5;54(2):1008-13. doi: 10.1167/iovs.12-10504.
There is growing evidence that disturbances in retinal oxygenation may trigger ocular diseases. New instruments allow for the noninvasive measurement of retinal oxygen saturation in humans. The present study was designed to investigate the retinal oxygen saturation in patients with chronic obstructive pulmonary disease (COPD). This was also done in an effort to test the validity of retinal oxygenation measurements with a retinal vessel analyzer.
In all, 16 patients with severe COPD grade 4 who were on long-term oxygen treatment were included in the study. For each patient two identical study days were scheduled. Measurements of retinal arterial and venous oxygen saturation were done using a commercially available instrument for retinal oxygen analysis. Peripheral arterial oxygen saturation values were analyzed with pulse oximetry and via a capillary blood sample drawn from the earlobe. Measurements were performed during oxygen treatment and during a period without oxygen supplementation. Analysis of all images for retinal oxygen saturation quantification was done by a masked investigator. Analysis was done using Pearson's correlation and a multivariate regression model.
Arterial and venous retinal oxygen saturation decreased significantly after the cessation of the oxygen therapy. The arteriovenous oxygen difference was unchanged while breathing ambient air or pure oxygen-enriched air. With both Pearson's correlation and the multivariate model, we found significant positive correlation coefficients between retinal arterial and peripheral arterial oxygen saturation as assessed with pulse oximetry as well as between retinal arterial and peripheral arterial oxygen saturation measured in blood samples. The change of oxygen saturation after discontinuation of oxygen supplementation showed a good correlation between retinal arterial oxygen saturation and peripheral arterial oxygen saturation (r = 0.53, P < 0.05). Reproducibility on the two study days was high.
The present study shows a good correlation between retinal arterial and peripheral arterial oxygen saturation indicating good validity of the technique. (ClinicalTrials.gov number, NCT00999024.).
越来越多的证据表明，视网膜供氧紊乱可能引发眼部疾病。新仪器可实现人类视网膜血氧饱和度的非侵入性测量。本研究旨在调查慢性阻塞性肺疾病（COPD）患者的视网膜氧饱和度。同时，还试图通过视网膜血管分析仪验证视网膜氧合测量的有效性。
共纳入 16 例长期氧疗的重度 COPD 4 级患者。为每位患者安排了两个相同的研究日。使用市售的视网膜氧分析仪器测量视网膜动脉和静脉血氧饱和度。通过脉搏血氧仪和耳垂毛细血管血样分析外周动脉血氧饱和度值。在氧疗期间和不补充氧气期间进行测量。由一名掩蔽研究者对所有视网膜氧饱和度定量图像进行分析。采用 Pearson 相关分析和多元回归模型进行分析。
停止氧疗后，视网膜动脉和静脉血氧饱和度显著降低。动静脉血氧差在呼吸环境空气或富氧空气时保持不变。通过 Pearson 相关分析和多元回归模型，我们发现视网膜动脉和脉搏血氧仪评估的外周动脉血氧饱和度之间以及视网膜动脉和血样测量的外周动脉血氧饱和度之间存在显著的正相关系数。停止氧补充后的氧饱和度变化与视网膜动脉氧饱和度和外周动脉氧饱和度之间具有良好的相关性（r=0.53，P<0.05）。两天研究的可重复性高。
本研究表明视网膜动脉和外周动脉血氧饱和度之间具有良好的相关性，表明该技术具有良好的有效性。（临床试验编号，NCT00999024.）。
