Yuasa S, Akagi R, Ubuka T
Department of Biochemistry, Okayama University Medical School, Japan.
Acta Med Okayama. 1990 Feb;44(1):47-50. doi: 10.18926/AMO/30463.
A method for the simultaneous determination of hypotaurine and taurine was developed. The method consisted of the elimination of urea, which interfered with the determination of hypotaurine, by immobilized urease, and determination of hypotaurine and taurine with an amino acid analyzer. The analyzer equipped with a cation-exchange column was operated at 32 degrees C with 0.2 M sodium citrate buffer, pH 2.8. Using this method, the dynamics of hypotaurine and taurine in blood plasma of rats was studied after the intraperitoneal injection of L-cysteine. The concentration of cysteine reached the maximum 1 h after L-cysteine loading. The concentration of hypotaurine and taurine increased in parallel and reached the maximum 2 h after L-cysteine loading. These changes seem to indicate the precursor-product relationship of these substances and the rapid conversion of hypotaurine to taurine in vivo.
建立了一种同时测定次牛磺酸和牛磺酸的方法。该方法包括通过固定化脲酶消除干扰次牛磺酸测定的尿素，并用氨基酸分析仪测定次牛磺酸和牛磺酸。配备阳离子交换柱的分析仪在32℃下用pH 2.8的0.2M柠檬酸钠缓冲液运行。使用该方法，研究了大鼠腹腔注射L-半胱氨酸后血浆中次牛磺酸和牛磺酸的动态变化。L-半胱氨酸负荷后1小时，半胱氨酸浓度达到最大值。次牛磺酸和牛磺酸浓度平行升高，并在L-半胱氨酸负荷后2小时达到最大值。这些变化似乎表明了这些物质之间的前体-产物关系以及次牛磺酸在体内迅速转化为牛磺酸。
