Futani S, Ubuka T, Abe T
Department of Biochemistry, Okayama University Medical School, Japan.
J Chromatogr B Biomed Appl. 1994 Oct 3;660(1):164-9. doi: 10.1016/0378-4347(94)00280-0.
A method for the determination of urinary hypotaurine and taurine for the purpose of studying hypotaurine-taurine status in mammals is described. Hypotaurine and taurine were converted into 4-dimethylaminoazobenzene-4'-sulfonyl (dabsyl) derivatives under conditions minimizing hypotaurine oxidation. The dabsylhypotaurine and dabsyltaurine formed were determined by reversed-phase HPLC. Average excretions of taurine and hypotaurine in rat urine were 270.5 and 2.5 mumol/kg body mass per day, respectively. After intraperitoneal injection of 5 mmol of L-cysteine/kg body mass, the increased excretions of taurine and hypotaurine corresponded to 22.1 and 2.5%, respectively, of L-cysteine administered, indicating that hypotaurine production exceeded the capacity of hypotaurine oxidation in vivo.
