Matsukawa S, Keil L C, Reid I A
Department of Physiology, University of California, San Francisco 94143.
Am J Physiol. 1990 Apr;258(4 Pt 2):F821-30. doi: 10.1152/ajprenal.1990.258.4.F821.
The observation that electrical stimulation of the renal nerves increases vasopressin secretion raises the possibility that the renal nerves may participate in the control of vasopressin secretion. In the present investigation, the effects of renal denervation on the vasopressin response to two reflex stimuli (nitroprusside infusion and hemorrhage) and two osmotic stimuli (hypertonic saline infusion and water deprivation) were studied in conscious, chronically prepared rabbits. Nitroprusside infusion in 13 intact and 14 denervated rabbits caused similar decreases in mean arterial pressure (MAP) and the increase in plasma arginine vasopressin concentration (PAVP) in intact (2.6 +/- 0.3 to 5.8 +/- 0.9 pg/ml, P less than 0.01) and denervated (2.8 +/- 0.3 to 5.7 +/- 1.3 pg/ml, P less than 0.01) rabbits was not significantly different. Hemorrhage (20 ml/kg) in 15 intact and 14 denervated rabbits caused similar decreases in MAP. Again, the increase in PAVP from 2.7 +/- 0.3 to 159.0 +/- 37.1 pg/ml (P less than 0.01) in intact and from 5.0 +/- 1.7 to 115.4 +/- 45.6 pg/ml (P less than 0.01) in denervated rabbits was not significantly different, nor was the relationship between PAVP and MAP in the two groups. In seven intact rabbits, hypertonic saline infusion increased PAVP from 4.0 +/- 0.9 to 10.9 +/- 2.8 pg/ml (P less than 0.05). The change in six denervated rabbits was not significantly different, nor was the relationship between PAVP and plasma osmolality. During water deprivation (24 h) in six intact rabbits, PAVP increased from 4.0 +/- 0.7 to 6.9 +/- 0.6 pg/ml (P less than 0.05). Again, the increase in PAVP in six denervated rabbits was not significantly different from that in the intact rabbits. The change in MAP during water deprivation in the two groups was also not significantly different. Renal cortical norepinephrine concentration in denervated kidneys was less than 10 ng/g wet wt. These results indicate that, in conscious rabbits, renal denervation does not impair the osmotic or reflex regulation of vasopressin secretion, nor does it interfere with blood pressure regulation during hypovolemia or hypotension.
电刺激肾神经会增加抗利尿激素分泌，这一观察结果提示肾神经可能参与抗利尿激素分泌的调控。在本研究中，我们在清醒、长期制备的家兔中，研究了肾去神经支配对两种反射刺激（硝普钠输注和出血）以及两种渗透压刺激（高渗盐水输注和禁水）引起的抗利尿激素反应的影响。13只完整家兔和14只去神经家兔输注硝普钠后，平均动脉压（MAP）均有相似程度的下降，完整家兔血浆精氨酸抗利尿激素浓度（PAVP）从2.6±0.3 pg/ml增至5.8±0.9 pg/ml（P<0.01），去神经家兔从2.8±0.3 pg/ml增至5.7±1.3 pg/ml（P<0.01），两者差异无统计学意义。15只完整家兔和14只去神经家兔出血（20 ml/kg）后，MAP均有相似程度的下降。同样，完整家兔PAVP从2.7±0.3 pg/ml增至159.0±37.1 pg/ml（P<0.01），去神经家兔从5.0±1.7 pg/ml增至115.4±45.6 pg/ml（P<0.01），两者差异无统计学意义，两组PAVP与MAP之间的关系也无显著差异。7只完整家兔输注高渗盐水后，PAVP从4.0±0.9 pg/ml增至10.9±2.8 pg/ml（P<0.05）。6只去神经家兔的变化无显著差异，PAVP与血浆渗透压之间的关系也无显著差异。6只完整家兔禁水24小时后，PAVP从4.0±0.7 pg/ml增至6.9±0.6 pg/ml（P<0.05）。同样，6只去神经家兔PAVP的增加与完整家兔无显著差异。两组禁水期间MAP的变化也无显著差异。去神经肾脏的肾皮质去甲肾上腺素浓度低于10 ng/g湿重。这些结果表明，在清醒家兔中，肾去神经支配不会损害抗利尿激素分泌的渗透压或反射调节，也不会干扰低血容量或低血压期间的血压调节。
