Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Syst Biol Reprod Med. 2013 Apr;59(2):117-22. doi: 10.3109/19396368.2012.760120. Epub 2013 Jan 11.
Among the different techniques available to evaluate blastocyst quality, the most frequently used are those that allow the counting of the number of cells of the two distinct cell lineages present at this stage (trophectoderm or TE and inner cell mass or ICM), through differential staining. The goal of this study was to compare three different methods for the differential staining of mouse blastocysts: a TE selective labelling method using a lectin, a TE permeabilization method based on the use of a detergent, and immunodetection of TE and ICM specific markers. Mouse blastocysts produced by parthenogenetic activation were used to determine and compare the efficiency and the cell counts of each method. The results showed that the TE permeabilization and immunodetection methods were superior, providing equivalent TE, ICM, and total cell counts.
在评估囊胚质量的不同技术中，最常用的技术是那些通过差异染色来计数此时存在的两个不同细胞谱系（滋养外胚层或 TE 和内细胞团或 ICM）的细胞数量的技术。本研究的目的是比较三种用于小鼠囊胚差异染色的不同方法：使用凝集素的 TE 选择性标记方法、基于去污剂使用的 TE 通透化方法以及 TE 和 ICM 特异性标记物的免疫检测。使用孤雌激活产生的小鼠囊胚来确定和比较每种方法的效率和细胞计数。结果表明，TE 通透化和免疫检测方法更优，提供了等效的 TE、ICM 和总细胞计数。
