Embryo Manipulation Laboratory, Animal Research Facility, Research Center For Translational Medicine, Koç University, Rumelifeneri yolu, 34450, Sariyer, Istanbul, Turkey.
Molecular Biology and Genetics Department, Faculty of Arts and Sciences, Haliç University, Haliç University, Sutluce Mah., Imrahor Cad., No:82, 34445, Istanbul, Turkey.
In Vitro Cell Dev Biol Anim. 2019 Aug;55(7):473-481. doi: 10.1007/s11626-019-00367-y. Epub 2019 Jun 18.
Leptin, a metabolic hormone, regulates the reproductive functions responding to both nutritional and body conditions. Embryonic stem cells play important roles in reproductive technology, but their derivation can be challenging. In this study, we evaluated the derivation rates of mouse embryonic stem cell (mESC) line from blastocysts developing in embryo culture media supplemented with different leptin concentrations. The results showed that addition of leptin into the embryo culture medium supported the in vitro development of mouse embryo. The mESC line derivation rates for media treated with 0, 10, 50, and 100 ng/ml of leptin were 61.24 % (54/88), 84.96 % (42/50), 81.79 % (61/76), and 85.78 % (56/67), respectively. In addition, leptin treatment of blastocysts upregulated the expression levels of the trophectoderm marker Cdx2, whereas inner cell mass markers Oct-4 and Nanog were not affected. mESC lines derived after leptin treatment demonstrated hallmarks of pluripotency, such as alkaline phosphatase activity, expression of, OCT4, NANOG, and SSEA1, as well as the ability to form embryoid bodies and well-differentiated teratomas. In conclusion, leptin has a positive effect on the derivation rate of mouse embryonic stem cell lines which may be, in part, due to its effects on the development of the trophectoderm cell lineage in the embryo.
瘦素是一种代谢激素，可响应营养和身体状况调节生殖功能。胚胎干细胞在生殖技术中发挥着重要作用，但它们的衍生可能具有挑战性。在这项研究中，我们评估了从添加不同瘦素浓度的胚胎培养介质中发育的囊胚中衍生出小鼠胚胎干细胞（mESC）系的衍生率。结果表明，在胚胎培养介质中添加瘦素可支持小鼠胚胎的体外发育。用 0、10、50 和 100ng/ml 的瘦素处理的培养基中 mESC 系的衍生率分别为 61.24%（54/88）、84.96%（42/50）、81.79%（61/76）和 85.78%（56/67）。此外，瘦素处理囊胚上调了滋养外胚层标志物 Cdx2 的表达水平，而对内细胞团标志物 Oct-4 和 Nanog 没有影响。经瘦素处理后衍生的 mESC 系表现出多能性的特征，如碱性磷酸酶活性、OCT4、NANOG 和 SSEA1 的表达，以及形成类胚体和分化良好的畸胎瘤的能力。总之，瘦素对小鼠胚胎干细胞系的衍生率具有积极影响，部分原因可能是其对胚胎滋养外胚层细胞系发育的影响。
