Department of Physiology, Faculty of Health Sciences, University of Pretoria, Private Bag x323, ARCADIA, 0007, South Africa.
Cardiovasc Pathol. 2013 May-Jun;22(3):241-2. doi: 10.1016/j.carpath.2012.11.005. Epub 2013 Jan 9.
BACKGROUND OR INTRODUCTION: Stroke is one of the most debilitating diseases causing morbidity and mortality worldwide. During ischemic stroke, erythrocytes undergo oxidative and proteolytic changes resulting not only in inflammation but also in changes in cellular rheology. During the event, fibrin fibers, which are typically a fine net, clot abnormally to form a clot of dense matted deposits (DMDs). This atypical coagulum causes blood cells to be trapped in the mesh.
Here we study red blood cell (RBC) ultrastructure in thromboembolic ischemic stroke using high resolution scanning electron microscopy.
We show that RBCs in patients change shape, with membrane extensions that form close interactions with the DMDs.
We suggest that the RBC membrane changes and resulting DMD interactions play a pivotal role in the persistent presence of thrombi. This ultrastructural observation might open a renewed debate regarding possible additional structural and biochemical roles of RBCs in the circulatory system.
目的：背景或引言：脑卒中是导致全世界发病率和死亡率的最具危害性的疾病之一。在缺血性脑卒中期间，红细胞发生氧化和蛋白水解变化，不仅导致炎症，而且还导致细胞流变学的变化。在此过程中，通常呈细网的纤维蛋白纤维异常凝结，形成致密的缠结沉积物（DMD）的凝块。这种非典型的凝结物导致血细胞被困在网眼中。
方法：在这里，我们使用高分辨率扫描电子显微镜研究血栓栓塞性缺血性脑卒中患者的红细胞（RBC）超微结构。
结果：我们发现患者的 RBC 形状发生变化，其膜延伸与 DMD 形成紧密的相互作用。
结论：我们认为 RBC 膜的变化和由此产生的 DMD 相互作用在血栓的持续存在中起关键作用。这种超微结构观察可能会重新引发关于 RBC 在循环系统中可能具有其他结构和生化作用的争论。
