Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Anal Chim Acta. 2013 Jan 25;761:194-200. doi: 10.1016/j.aca.2012.11.046. Epub 2012 Dec 7.
A new kind of ultrabright fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle (FCMSN) is reported. A luminescent dye, Rhodamine 6G or tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), is doped inside nanochannels of a silica matrix. The hydrophobic groups in the silica matrix avoid the leakage of dye from open channels. The amines groups on the surface of the FCMSN improve the modification performance of the nanoparticle. Because the nanochannels are isolated by a network skeleton of silica, fluorescence quenching based on the inner filter effect of the fluorescent dyes immobilized in nanochannels is weakened effectively. The Quantum Yield of obtained 90 nm silica particles was about 61%. Compared with the fluorescent core-shell nanoparticle, the chemiluminescence reagents can freely enter the nanoparticles to react with fluorescent dyes to create chemiluminescence. The results show that the FCMSN are both fluorescent labels and chemiluminescent labels. In biological applications, the NaIO(4) oxidation method was proven to be superior to the glutaraldehyde method. The amount of amino could affect the specificity of the FCMSN. The fluorescence microscopy imaging demonstrated that the FCMSN is viable for biological applications.
一种新型的超亮荧光和化学发光双功能介孔硅纳米粒子（FCMSN）被报道。一种发光染料，若丹明 6G 或三（2,2'-联吡啶）二氯化钌（II）六水合物（Rubpy），被掺杂在硅基质的纳米通道内。硅基质中的疏水区避免了染料从开放通道中泄漏。FCMSN 表面的胺基提高了纳米粒子的修饰性能。由于纳米通道被硅的网络骨架隔离，固定在纳米通道中的荧光染料的内滤效应引起的荧光猝灭被有效减弱。得到的 90nm 硅粒子的量子产率约为 61%。与荧光核壳纳米粒子相比，化学发光试剂可以自由进入纳米粒子与荧光染料反应产生化学发光。结果表明，FCMSN 既是荧光标记物又是化学发光标记物。在生物应用中，NaIO(4)氧化法被证明优于戊二醛法。氨基的数量会影响 FCMSN 的特异性。荧光显微镜成像表明，FCMSN 适用于生物应用。
