舌部间叶性软骨黏液样肿瘤：对组织发生学的认识。

Ectomesenchymal chondromyxoid tumor of the tongue: insights on histogenesis.

机构信息

Department of Molecular Oral Medicine and Maxillofacial Surgery, Applied Life Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Feb;115(2):233-40. doi: 10.1016/j.oooo.2012.11.008.

DOI:10.1016/j.oooo.2012.11.008

PMID:23312916

Abstract

OBJECTIVE

The objective of this study was to investigate the histogenesis of ectomesenchymal chondromyxoid tumors (ECTs) of the tongue.

STUDY DESIGN

The biochemical characteristics of a rarely occurring tumor of the tongue were analyzed by immunohistochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR), and its biological properties were assessed in primary culture in serum-free media.

RESULTS

Immunohistochemistry showed that the tumor cells were strongly positive for vimentin, S-100, and glial fibrillary acidic protein (GFAP), but negative for cytokeratin and epithelial membrane antigen. In primary cultures, the cells derived from the ECT were morphologically similar to neuronal cells and expressed Nanog, GFAP, and MAP2. RT-PCR analysis of the surgical specimen was positive for OCT3/4, Sox2, Nanog, MAP2, and CD105 mRNAs.

CONCLUSIONS

The results of the present study indicate that ECTs originate from the ectomesenchymal cells of the neural crest and are similar in their molecular and biological characteristics to undifferentiated mesenchymal stem cells.

摘要

目的

本研究旨在探讨舌部间叶性软骨黏液样肿瘤（ECT）的组织发生。

研究设计

通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）分析一种罕见舌部肿瘤的生化特征，并在无血清培养基中进行原代培养评估其生物学特性。

结果

免疫组织化学显示肿瘤细胞强烈表达波形蛋白、S-100 和神经胶质纤维酸性蛋白（GFAP），但不表达细胞角蛋白和上皮膜抗原。在原代培养中，源自 ECT 的细胞形态上类似于神经元细胞，并表达 Nanog、GFAP 和 MAP2。手术标本的 RT-PCR 分析显示 OCT3/4、Sox2、Nanog、MAP2 和 CD105mRNA 呈阳性。