Department of Otolaryngology, Oita University Faculty of Medicine, Oita, Japan.
Ultrasound Med Biol. 2013 Mar;39(3):413-23. doi: 10.1016/j.ultrasmedbio.2012.10.003. Epub 2013 Jan 11.
We hypothesized that low-intensity focused ultrasound (LIFU) increases vessel permeability and antibacterial drug activity in the mouse middle ear. We determined appropriate settings by applying LIFU to mouse ears with the external auditory canal filled with normal saline and performed histologic and immunohistologic examination. Acute otitis media was induced in mice with nontypable Haemophilus influenzae, and they were given ampicillin (50, 10, or 2 mg/kg) intraperitoneally once daily for 3 days with or without LIFU (1.0 W/cm(2), 20% duty cycle, 30 s). In the LIFU(+) groups receiving the 2- and 10-mg/kg doses, viable bacteria counts, number of inflammatory cells and IL-1β and TNF-α levels in middle ear effusion were significantly lower than in the LIFU(-) groups on the same doses. Severity of AOM also tended to be reduced more in the LIFU(+) groups than in the LIFU(-) groups. LIFU application with antibiotics may be effective for middle ear infection.
我们假设低强度聚焦超声（LIFU）可增加小鼠中耳血管通透性和抗菌药物活性。我们通过将 LIFU 施加到充满生理盐水的外耳道口的小鼠耳朵上来确定适当的设置，并进行组织学和免疫组织化学检查。用非典型流感嗜血杆菌诱导小鼠急性中耳炎，并给予氨苄西林（50、10 或 2 mg/kg）腹膜内注射，每日一次，共 3 天，同时或不使用 LIFU（1.0 W/cm(2)，20%占空比，30 s）。在接受 2 和 10 mg/kg 剂量的 LIFU(+)组中，与相同剂量的 LIFU(-)组相比，中耳积液中的活菌计数、炎症细胞数量以及 IL-1β 和 TNF-α 水平显著降低。LIFU(+)组的 AOM 严重程度也倾向于比 LIFU(-)组降低更多。抗生素联合 LIFU 应用可能对中耳感染有效。
