College of Science, Biology Department, Sultan Qaboos University, Oman.
J Microbiol Biotechnol. 2013 Jan;23(1):106-17. doi: 10.4014/jmb.1204.04021.
Microbial enhanced oil recovery (MEOR) is one of the most economical and efficient methods for extending the life of production wells in a declining reservoir. Microbial consortia from Wafra oil wells and Suwaihat production water, Al-Wusta region, Oman were screened. Microbial consortia in brine samples were identified using denaturing gradient gel electrophoresis and 16S rRNA gene sequences. The detected microbial consortia of Wafra oil wells were completely different from microbial consortia of Suwaihat formation water. A total of 33 genera and 58 species were identified in Wafra oil wells and Suwaihat production water. All of the identified microbial genera were first reported in Oman, with Caminicella sporogenes for the first time reported from oil fields. Most of the identified microorganisms were found to be anaerobic, thermophilic, and halophilic, and produced biogases, biosolvants, and biosurfactants as by-products, which may be good candidates for MEOR.
微生物强化采油(MEOR)是延长衰竭油藏中生产井寿命的最经济、最有效的方法之一。对来自阿曼瓦尔法地区苏海特生产水和 Wafra 油井的微生物菌群进行了筛选。采用变性梯度凝胶电泳和 16S rRNA 基因序列对盐水中的微生物菌群进行了鉴定。Wafra 油井中的检测到的微生物菌群与苏海特地层水的微生物菌群完全不同。在 Wafra 油井和苏海特生产水中共鉴定出 33 属和 58 种。在阿曼首次报道了所有鉴定出的微生物属,首次从油田中报道了 sporogenes 属的 Caminicella。大多数鉴定出的微生物是厌氧菌、嗜热菌和嗜盐菌,作为副产物产生生物气、生物溶剂和生物表面活性剂,它们可能是 MEOR 的良好候选物。