Microbial Biotechnology, Environmental and Industrial Biotechnology Division, The Energy and Resources Institute (TERI), New Delhi, India.
Microbial Biotechnology, Environmental and Industrial Biotechnology Division, The Energy and Resources Institute (TERI), New Delhi, India.
J Biotechnol. 2018 Oct 10;283:43-50. doi: 10.1016/j.jbiotec.2018.06.347. Epub 2018 Jul 1.
In past years, lots of research has been focused on the indigenous bacteria and their mechanisms, which help in enhanced oil recovery. Most of the oil wells in Indian subcontinent have temperature higher than 60 °C. Also, the role of methanogenic consortia from high temperature petroleum reservoir for enhanced oil recovery (EOR) has not been explored much. Hence, in the present study methanogens isolated from thermophilic oil wells (70 °C) were evaluated for enhanced oil recovery. Methane gas is produced by methanogens, which helps in oil recovery from depleted oil wells through reservoir re-pressurization and also can be recovered from reservoir along with crude oil as alternative energy source. Therefore, in this study indigenous methanogenic consortium (TERIL146) was enriched from high temperature oil reservoir showing (12 mmol/l) gas production along with other metabolites. Sequencing analysis revealed the presence of Methanothermobacter sp., Thermoanaerobacter sp., Gelria sp. and Thermotoga sp. in the consortium. Furthermore, the developed indigenous consortium TERIL146 showed 8.3% incremental oil recovery in sandpack assay. The present study demonstrates successful recovery of both oil and energy (gas) by the developed indigenous methanogenic consortium TERIL146 for potential application in thermophilic depleted oil wells of Indian subcontinent.
在过去的几年中，大量的研究集中在土著细菌及其机制上，这些机制有助于提高石油采收率。印度次大陆的大多数油井温度都高于 60°C。此外，高温石油储层中产甲烷菌菌群在提高石油采收率（EOR）中的作用尚未得到充分探索。因此，本研究从嗜热油井（70°C）中分离出产甲烷菌，评估其对提高石油采收率的作用。产甲烷菌会产生甲烷气体，通过储层再增压帮助从枯竭油井中回收石油，也可以与原油一起从储层中回收，作为替代能源。因此，在这项研究中，从产气量为（12mmol/l）的高温油藏中富集了土著产甲烷菌菌群（TERIL146）以及其他代谢物。测序分析显示，该菌群中存在 Methanothermobacter sp.、Thermoanaerobacter sp.、Gelria sp. 和 Thermotoga sp.。此外，在填砂模型实验中，开发的土著菌群 TERIL146 可提高 8.3%的采油量。本研究表明，开发的土著产甲烷菌菌群 TERIL146 可成功回收石油和能源（天然气），有望应用于印度次大陆的高温枯竭油井。
